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Applied Microbiology and Biotechnology

, Volume 44, Issue 1–2, pp 106–111 | Cite as

Cellulase induction by lactose in Trichoderma reesei PC-3-7

  • Y. Morikawa
  • T. Ohashi
  • O. Mantani
  • H. Okada
Applied Genetics and Regulation

Abstract

In an attempt to clarify the function of lactose in cellulase induction, experiments were carried out on cellulase formation by lactose along with other sugars in a resting cell system of Trichoderma reesei PC-3-7, a hypercellulase-producing mutant. Although lactose alone induces little cellulase under the conditions used, a synergistic effect on cellulase formation was observed following the respective addition of sophorose, cellobiose or galactose to lactose. The lactose consumption was more rapid when these sugars were added than in their absence. Furthermore, following lactose addition 10 h after the beginning of cultivation in the presence of cellobiose, cellulase formation was initiated with only a little lag, and lactose consumption started immediately, being complete in 14 h. \-Galactosidase induction experiments suggested that the rapid consumption of lactose is possibly not dependent on lactose degradation by the enzyme. From these results, it is suggested that lactose may function as an inducer for cellulase formation if it is taken up in the mycelium of T. reesei PC-3-7, and that sophorose, cellobiose or galactose may induce a putative lactose permease. *** DIRECT SUPPORT *** AG903066 00005

Keywords

Enzyme Sugar Cellulase Lactose Galactose 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Y. Morikawa
    • 1
  • T. Ohashi
    • 1
  • O. Mantani
    • 1
  • H. Okada
    • 1
  1. 1.Department of BioengineeringNagaoka University of TechnologyNagaoka, NiigataJapan

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