Applied Microbiology and Biotechnology

, Volume 44, Issue 1–2, pp 53–58

Improved yields of the extracellular domain of the epidermal growth factor receptor produced using the baculovirus expression system by medium replacement following infection

  • R. L. Tom
  • M. T. Debanne
  • C. Bédard
  • A. W. Caron
  • B. Massie
  • A. A. Kamen
Biotechnology

DOI: 10.1007/BF00164480

Cite this article as:
Tom, R.L., Debanne, M.T., Bédard, C. et al. Appl Microbiol Biotechnol (1995) 44: 53. doi:10.1007/BF00164480

Abstract

The extracellular domain of the epidermal growth factor receptor (EGFR) was expressed using the baculovirus expression vector system. The maximum level of the EGFR extracellular domain secreted into the medium in Sf-9 (Spodoptera frugiperda or fall armyworm) cell batch culture was approximately 2.5 μg ml−1. In order to increase this yield, a process was developed that included the following sequence of steps: batch growth to maximum cell density, infection of the cells with recombinant virus, and replacement of spent medium. By using this process, the specific yield of recombinant protein, which in batch culture drops when infection is carried out at densities greater than 3 × 106 cells ml−1, can be maintained at a maximum in cultures infected at densities of 107 cells ml−1 or greater. The process, when applied to 3-1 and 11-1 bioreactor cultures, allowed a maximum volumetric yield of triple the maximum value attainable in batch culture. Spent-medium analysis indicates that medium replacement provides certain nutrients that could otherwise be limiting for recombinant protein production.

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • R. L. Tom
    • 1
  • M. T. Debanne
    • 2
  • C. Bédard
    • 1
  • A. W. Caron
    • 1
  • B. Massie
    • 1
  • A. A. Kamen
    • 1
  1. 1.Animal Cell Engineering Group, Biotechnology Research InstituteNational Research Council of CanadaMontréal QuébecCanada
  2. 2.Cell Surface Recognition Group, Biotechnology Research InstituteNational Research Council of CanadaMontréal QuébecCanada

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