Applied Microbiology and Biotechnology

, Volume 44, Issue 1–2, pp 15–19

Fermentation of glycerol to 1,3-propanediol: use of cosubstrates

  • H. Biebl
  • S. Marten
Biotechnology

Abstract

Three fermentable substances, glucose, 1,2-ethanediol and 1,2-propanediol were checked as cosubstrates for the fermentation of glycerol by Clostridium butyricum and Citrobacter freundii with the aim of achieving a complete conversion of glycerol to 1,3-propanediol. Glucose was fermented by C. butyricum mainly to acetate, CO2 and reducing equivalents in the presence of glycerol and contributed markedly to the 1,3-propanediol yield. However, because of relatively slow growth on glucose, complete conversion was not achieved. If the two glycols were used as cosubstrates for glycerol fermentation, the 1,3-propanediol yield did not increase but dimished considerably, as they were converted to more reduced products, i.e. alcohols instead of acids. From 1,2-propanediol 2-propanol was formed in addition to 1-propanol. The ratio of the propanols was dependent on the culture conditions.

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References

  1. Azova LG (1967) Utilization of glycerol by some strains of butyric acid bacteria. Doklady Akad Nauk SSSR 172:1434–1435Google Scholar
  2. Biebl H (1991) Glycerol fermentation to 1,3-propanediol by Clostridium butyricum. Measurement of product inhibition by use of a pH-auxostat. Appl Microbiol Biotechnol 35:701–705Google Scholar
  3. Biebl H, Marten S, Hippe H, Deckwer W-D (1992) Glycerol conversion to 1,3-propanediol by newly isolated clostridia. Appl Microbiol Biotechnol 36:592–597Google Scholar
  4. Forage RG, Foster MA (1979) Resolution of the coenzyme B12-dependent dehydratases of Klebsiella sp. and Citrobacter freundii. Biochim Biophys Acta 569:249–258Google Scholar
  5. Forsberg CW (1987) Production of 1,3-propanediol from glycerol by Clostridium acetobutylicum and other Clostridium species. Appl Environ Microbiol 53:639–643Google Scholar
  6. Gaston LW, Stadtman ER (1963). Fermentation of ethylene glycol by Clostridium glycolicum sp. n. J Bacteriol 85:356–362Google Scholar
  7. Gottschalk G, Averhoff B (1988) Process for the microbiological preparation of 1,3-propandiol from glycerol. European patent no. 0 373 230 A1Google Scholar
  8. Günzel B, Yonsel S, Deckwer W-D (1991) Fermentative production of 1,3-propanediol from glycerol by Clostridium butyricum up to a scale of 2 m3. Appl Microbiol Biotechnol 36:289–295Google Scholar
  9. Hartmannis MGN, Stadtman T (1986) Diol metabolism and diol dehydratase in Clostridium glycolicum. Arch Biochem Biophys 245:144–152Google Scholar
  10. Heyndrickx M, Vos P de, Vancanneyt M, Ley J de (1991) The fermentation of glycerol by Clostridium butyricum LMG 1212 t2 and 1213 t1 and C. pasteurianum LMG 3285. Appl Microbiol Biotechnol 34:637–642Google Scholar
  11. Homann T, Tag C, Biebl H, Deckwer W-D, Schink B (1990) Fermentation of glycerol to 1,3-propanediol by Klebsiella and Citrobacter strains. Appl Microbiol Biotechnol 33:121–126Google Scholar
  12. Hoshino K (1960) Studies on an organism producing isopropanol from acetone. Part V. Enzymological study on the oxidation-reduction of Lactobacillus brevis var. hofuensis (in Japanese). Nippon Nogei kagaku Kaishi 34:608–615Google Scholar
  13. Mickelson MN, Werkman CH (1940) The dissimilation of glycerol by coli-aerogenes intermediates. J Bacteriol 39:709–715Google Scholar
  14. Nakas JP, Schaedle M, Parkinson CM, Coonley CE, Tanenbaum SW (1983) System development of linked fermentation production of solvents from algal biomass. Appl Environ Microbiol 46:1017–1023Google Scholar
  15. Schink B, Stieb M (1983) Fermentative degradation of polyethylene glycol by a strictly anaerobic, gram-negative, nonsporeforming bacterium, Pelobacter venetianus sp. nov. Appl Environ Microbiol 45:1905–1913Google Scholar
  16. Tong I-T, Cameron D (1992) Enhancement of 1,3-propanediol production by cofermentation in Escherichia coli expressing Klebsiella pneumoniae dha regulon genes. Appl Biochem Biotechnol 34/35:149–159Google Scholar
  17. Toraya T, Honda S, Fukui S (1979) Fermentation of 1,2-propanediol and 1,2-ethanediol by some genera of Enterobacteriaceae, involving coenzyme B12-dependent diol dehydratase. J Bacteriol 139:39–47Google Scholar
  18. Tran-Dinh K, Hill, FF (1987) Verfahren zur Herstellung von Propan diol-(1,3). German patent no. DE 37 34 764 A1Google Scholar
  19. Vasconcelos I, Girbal L, Soucaille P (1994) Regulation of carbon and electron flow in Clostridium acetobutylicum grown in chemostat culture at neutral pH on mixtures of glucose and glycerol. J Bacteriol 176:1443–1450Google Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • H. Biebl
    • 1
  • S. Marten
    • 1
  1. 1.Gesellschaft für Biotechnologische Forschung mbHBraunschweigGermany

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