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Degradation of 4′-dimethylaminoazobenzene-2-carboxylic acid by Pseudomonas stutzeri

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Abstract

4′-Dimethylaminoazobenzene-2-carboxylic acid (DMBC) was utilized as a necessary carbon and nitrogen source by Pseudomonas stutzeri IAM 12097. o-Aminobenzoic acid (o-ABA), N,N-dimethyl-p-phenylenediamine (DMPA) and cathecol were identified as intermediates of DMBC degradation. DMBC was degraded at a concentration below 70 μmol dm−3. The ability to utilize DMBC in P. stutzeri was lost spontaneously to some extent. When P. stutzeri was cured of plasmid DNA (approximately 8 MDal) by treatment with mitomycin C, acridine orange, and chloramphenicol, DMBC was not utilized by the resultant strain. These facts suggest that the degradative ability on DMBC in P. stutzeri is controlled by plasmid DNA.

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References

  • Bertani G (1951) Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherihia coli. J Bacteriol 62:293–300

    Google Scholar 

  • Hirano K, Mori K, Tsuboi N, Kawai S, Ohno T (1972) Gas chromatography of urinary anthranilic acid and 3-hydroxyanthranilic acid by solvent extraction method. Chem Pharm Bull 20:1412–1416

    Google Scholar 

  • Huang M-T, Miwa GT, Lu AYH (1978) Induction of rat liver cytosol methyl red azo-reductase by 3-methylcholanthrene assayed by a sensitive fluorometric method. Biochem Biophys Res Commun 83:1253–1259

    Google Scholar 

  • Kado CI, Liu ST (1981) Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol 145:1365–1373

    Google Scholar 

  • Kulla HG, Klausener F, Meyer U, Lüdeke B, Lsisinger T (1983) Interference of aromatic sulfo groups in the microbial degradation of the azo dyes Orange I and Orange II. Arch Microbiol 135:1–7

    Google Scholar 

  • Mechsner K, Wuhrmann K (1982) Cell permeability as a rate limiting factor in the microbial reduction of sulfonated azo dyes. Eur J Appl Microbiol Biotechnol 15:123–126

    Google Scholar 

  • Wurhmann K, Mechsner KI, Kappeler Th (1980) Investigation on rate-determining factors in the microbial reduction of azo dyes. Eur J Appl Microbiol Biotechnol 9:325–338

    Google Scholar 

  • Zimmermann T, Kulla HG, Leisinger T (1982) Properties of purifified Orange II azoreductase, the enzyme initiating azo dye degradation by Pseudomonas KF 46. Eur J Biochem 129:197–203

    Google Scholar 

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Correspondence to: C. Yatome

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Yatome, C., Matsufuru, H., Taguchi, T. et al. Degradation of 4′-dimethylaminoazobenzene-2-carboxylic acid by Pseudomonas stutzeri . Appl Microbiol Biotechnol 39, 778–781 (1993). https://doi.org/10.1007/BF00164466

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  • DOI: https://doi.org/10.1007/BF00164466

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