Journal of Molecular Evolution

, Volume 40, Issue 4, pp 400–404 | Cite as

Sequence characteristics of a cervid DNA repeat family

  • Sohail A. Qureshi
  • R. D. Blake
Articles

Abstract

The (G + C) distribution and the presence and amounts of repetitive sequence families in the white-tailed deer (Odocoileus virginianus) have been examined. The distribution ranges from 20 to 70% (G + C) and shows four distinct repeat families. A 0.7-kb family, DII, corresponds to satellite II in domestic bovids—ox, sheep, and goat—and was singled out for detailed characterization. DII has a prototypic repeat of 67% (G + C), consists of 25,000 tandem copies, and contributes 1.7% to the genomic DNA. Sequencing and electrophoretic analysis indicate a repeat length of 691 bp. These characteristics are similar to those of the bovid satellite II families as well as to those of other cervids that we have examined. The intraspecific sequence divergence within this family has a variance of only 2.5 ± 0.3%.

Key words

Satellite sequences Artiodactyla White-tailed deer Cervid Satellite II 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Allard MW, Miyamoto MM, Jerecki L, Kraus F, Tennant MR (1992) DNA systematics and evolution of the artiodactyl family bovidea. Proc Natl Acad Sci USA 89:3972–3976Google Scholar
  2. Blake RD, Lefoley SG (1978) Spectral analysis of high-resolution direct-derivative melting curves of DNA for instantaneous and total base composition. Biochim Biophys Acta 518:233–246Google Scholar
  3. Blake RD, Hydorn TG (1985) Spectral analysis for base composition of DNA undergoing melting. J Biochem Biophys Methods 11:307–316Google Scholar
  4. Blake RD (1987) Cooperative lengths of DNA during melting. Biopolymers 26:1063–1074Google Scholar
  5. Bonner T, Brenner D, Neufeld B, Britten R (1973) Reduction in the rate of DNA reassociation by sequence divergence. J Mol Biol 81:123–135Google Scholar
  6. Buckland RA (1983) Comparative structure and evolution of goat and sheep satellite-II DNAs. Nucleic Acids Res 11:1349–1360Google Scholar
  7. Buckland RA (1985). Sequence and evolution of related bovine and caprine satellite DNAs. J Mol Biol 186:25–30Google Scholar
  8. Buckland RA, Elder JK (1985) On the mechanism of amplification of satellite II DNA sequences of the domestic goat Capra hircus. J Mol Biol 186:12–23Google Scholar
  9. Gentry AW, Hooker JJ (1988) The phylogeny of the artiodactyla. In: Benton MJ (ed) The phylogeny and classification of the tetrapods, vol 2. Clarendon Press, Oxford, pp 235–272Google Scholar
  10. Irwin DM, Wilson AC (1990) Concerted evolution of ruminant stomach lysozymes. J Biol Chem 265:4944–4952Google Scholar
  11. Janis CM, Scott KM (1988) The phylogeny of the ruminantia (Artiodactyla, Mammalia). In: Benton MJ (ed) The phylogeny and classification of the tetrapods, vol. 2. Clarendon Press, Oxford, pp 273–282Google Scholar
  12. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, NYGoogle Scholar
  13. Miyamoto MM, Goodman M (1986) Biomolecular systematics of eutherian mammals: phylogenetic patterns and classification. Syst Zool 35:230–240Google Scholar
  14. Miyamoto MM, Kraus F, Ryder OA (1990) Phylogeny and evolution of antlered deer determined from mitochondrial DNA sequences. Proc Natl Acad Sci USA 87:6127–6131Google Scholar
  15. Novacek MJ (1982) Information for molecular studies from anatomical and fossil evidence on higher eutherian phylogeny. In: Goodman M (ed) Macromolecular sequences in systematic and evolutionary biology. Plenum Press, NY, pp 3–41Google Scholar
  16. Pearson WR, Lipman DJ (1988) Improved tools for biological sequence comparison. Proc Natl Acad Sci USA 87:6127–6131Google Scholar
  17. Qureshi SA (1990) Characterization of satellite DNAs from selected artiodactyla and their potential as evolutionary clocks. PhD Dissertation, University of MaineGoogle Scholar
  18. Romer AS (1966) Vertebrate paleontology, 3rd ed. University of Chicago Press, ChicagoGoogle Scholar
  19. Romer R, Riesner D, Courts SM, Maass G (1970) The coupling of conformational transitions in t-RNA from yeast studied by a modified differential absorption technique. Eur J Biochem 15:77–84Google Scholar
  20. Romero-Herrera AE, Lehman H, Joysey KA, Friday AE (1973) Molecular evolution of myoglobin and the fossil record: a phylogenetic synthesis. Nature 246:389–395Google Scholar
  21. Springer MS, Davidson EH, Britten RJ (1992) Calculation of sequence divergence from the thermal stability of DNA heteroduplexes. J Mol Evol 34:379–382Google Scholar
  22. Vizard DL, White RA, Ansevin AT (1984) An analysis of repeated sequence heterogeneity. Arch Biochem Biophys 229:498–508Google Scholar
  23. Wilson AC, Cann RL, Carr SM, George M, Gillensten UB, Helm-Bychowski KM, Higuchi G, Palumbi SR, Prager EM, Sage RD, Stoneking M (1985) Mitochondrial DNA and two perspectives on evolutionary genetics. Biol J Linn Soc 26:375–400Google Scholar
  24. Wilson AC, Ochman H, Prager EM (1987) Molecular time scale for evolution. Trends Genet 3:241–247Google Scholar
  25. Zhang H, Scholl R, Browse J, Somerville C (1988) Double stranded DNA sequencing as a choice for DNA sequencing. Nucleic Acids Res 16:1220Google Scholar

Copyright information

© Springer-Verlag New York Inc 1995

Authors and Affiliations

  • Sohail A. Qureshi
    • 1
  • R. D. Blake
    • 1
  1. 1.Department of Biochemistry, Microbiology, and Molecular BiologyUniversity of MaineOronoUSA

Personalised recommendations