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Biotechnology Techniques

, Volume 4, Issue 2, pp 107–112 | Cite as

Extraction and quantitation of astaxanthin from Phaffia rhodozyma

  • J. James Sedmak
  • Deepthi K. Weerasinghe
  • Setsuko O. Jolly
Assay Methods

Summary

The rapid, quantitative release of astaxanthin and other carotenoids from the yeast Phaffiarhodozyma is described. Hashed cells are ruptured with dimethylsulfoxide (DMSO) and carotenoids extracted into an organic solvent. Extraction and spectrophotometric quantitation of total carotenoids is rapid, reproducible and only small volumes (0.1–2 ml) of culture are required. HPLC analysis in normal phase silica gel column indicates that astaxanthin comprises 65–95% of the total pigmented carotenoids of P.rhodozyma.

Keywords

HPLC DMSO Carotenoid Dimethylsulfoxide Small Volume 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. An, G.-H., Schuman, D.B., and Johnson, E.A. (1989) Applied Environ. Microbiol. 55, 116–124.Google Scholar
  2. Haard, N.F. (1988) Biotech. Letters 10, 609–614.Google Scholar
  3. Ruddat, M., and Will O.H. III (1985) Meth. Enzymol. 111, 189–200.Google Scholar
  4. Torrissen, O.J. (1986) Aquaculture 53, 271–278.Google Scholar

Copyright information

© Chapman & Hall 1990

Authors and Affiliations

  • J. James Sedmak
    • 1
  • Deepthi K. Weerasinghe
    • 1
  • Setsuko O. Jolly
    • 1
  1. 1.Molecular Biology DepartmentUniversal Foods CorporationMilwaukeeUSA

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