Advertisement

Biotechnology Techniques

, Volume 8, Issue 3, pp 209–213 | Cite as

Plasmid elimination from clinical isolates of Echerichia coli by ciprofloxacin and other inhibitors of DNA gyrase

  • Aditya. B. Pant
  • G. S. Randhawa
  • G. D. Sharma
  • M. K. Kapil
Article
  • 34 Downloads

Summary

The susceptibility of 50 drug resistant strains of Escherichia coli of human gut was determined against ciprofloxacin, acridine orange (AO) and sodium dodecyl sulphate (SDS). Curing efficacy of these agents were worked out at subminimal inhibitory concentrations. Ciprofloaxacin was found a better curing agent for E coli R-plasmids, eliminating R-factors from 48% of the strains followed by SDS and AO which eliminated 24% and 20% of the drug resistance determinants, respectively. Elimination of R-plasmids was found dependent on the concentrations of curing agents and nature of R-plasmids.

Keywords

Sodium Dodecyl Sulphate Drug Resistance Inhibitory Concentration Clinical Isolate Resistant Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Ahmad, S., J.N.S. Yadava (1988) Ind. J. Exp. Biol. 26(II): 601–605.Google Scholar
  2. 2.
    Attfield, P.V., R.J. Pinney (1985) Antimicro. Agents. Chemother. 27: 985–988.Google Scholar
  3. 3.
    Bazzicaluppo, P., G.P. Touhini-valentini (1972) Proc. Natl. Acad. Sci.USA, 69: 298–300.Google Scholar
  4. 4.
    Bounachaud, D.H., M.R. Scavizzi., Y.A. chabbert (1969) J. Gen. Microbiol. 54: 417–425.Google Scholar
  5. 5.
    Bridge W.P., J.D. Manning (1983) New. Med. J. 96: 418–420.Google Scholar
  6. 6.
    Carbonetti, N.H., P.H. Williams (1984) Infect. Immun. 46: 7–12.Google Scholar
  7. 7.
    Courvalin, P (1990) Antimicro. Chemother. 34 (5): 681–684.Google Scholar
  8. 8.
    Danilevskaya, O.N., A.I. Gragerov (1980) J. Molecular Gen-Genet. 178: 233–235.Google Scholar
  9. 9.
    Gelbert, M., M.H. O'Dla., T. Itoh., J.I. Tomizawa (1976) Proc. Natl. Acad. Sci. USA, 73: 4474–4478.Google Scholar
  10. 10.
    Hohn, F.E., J. Clak (1976), Antimicro. Agent. Chemother. 9: 77–80.Google Scholar
  11. 11.
    Hirai, K., T. Irikura, S. Iyobe (1984) Chemothesapy. 32: 471–478.Google Scholar
  12. 12.
    Hirota, Y (1960) Proc. Natl. Acad. Sci. USA, 46: 5764.Google Scholar
  13. 13.
    Hooper, D.C., J.S. Wolfron, G.L. Mchugh (1984) Antimicro. Agent. Chemother 25: 586–590.Google Scholar
  14. 14.
    Iyer, V.N., W. Szy balski (1964) Science 145.Google Scholar
  15. 15.
    Kaur, P., M. Saxena, D.V. Vadehra (1985) Ind. J. Med. Res. 82:122–126.Google Scholar
  16. 16.
    Ledeberg, J., E.M. Lederberg (1952) J. Bacteriol. 63: 399–406.Google Scholar
  17. 17.
    Moegel, A., U. Rdest, W. Goebel (1981) J. Bacteriol. 45:233–247.Google Scholar
  18. 18.
    Peter, H., H. Schedletzley, H. Paul (1993) Antimicro. Agent. Chemother. 37 (4): 696–701.Google Scholar
  19. 19.
    Singh, M., J.N.S. Yadava. (1988). Ind. J. Exp. Biol. 26:149–154.Google Scholar
  20. 20.
    Smith, H.W. (1963) J. Path. Bacteriol. 85: 187–211.Google Scholar
  21. 21.
    Sugino, A., C.I. Peebles, K.N. Krenzer, N.R. Cozzarelli (1977) Proc. Natl. Acad. Sci. USA. 74: 4767–4771.Google Scholar
  22. 22.
    Watanabe, T. T. Fukasawa (1961-b) J. Bacteriol. 81:679–683.Google Scholar
  23. 23.
    Weisser, J., B, Wiedemann. (1985) Antimicro. Agent. Chemother. 28:700–702.Google Scholar
  24. 24.
    Weisser, J., B. Weidemann. (1986). J. Antimicro. chamother. 18:575–581.Google Scholar
  25. 25.
    Yosida, H., M. Makamusa, M. Bogaki, Hideakiito, T. Kojima, H. Hattoria and S.Nakamura (1993) Antimicro. Agent. Chemother. 37(4) 839–845.Google Scholar

Copyright information

© Science & Technology Letters 1994

Authors and Affiliations

  • Aditya. B. Pant
    • 1
  • G. S. Randhawa
    • 1
  • G. D. Sharma
    • 2
  • M. K. Kapil
    • 2
  1. 1.Department of Biosciences & BiotechnologyUniversity of RoorkeeRoorkeeIndia
  2. 2.Division of BiochemistryCentral Drug Research InstituteLucknowIndia

Personalised recommendations