Journal of Molecular Evolution

, Volume 35, Issue 4, pp 318–328 | Cite as

The nucleotide sequence of the entire ribosomal DNA operon and the structure of the large subunit rRNA of Giardia muris

  • Harry van Keulen
  • Robin R. Gutell
  • Scott R. Campbell
  • Stanley L. Erlandsen
  • Edward L. Jarroll
Article

Summary

The total nucleotide sequence of the rDNA of Giardia muris, an intestinal protozoan parasite of rodents, has been determined. The repeat unit is 7668 basepairs (bp) in size and consists of a spacer of 3314 bp, a small-subunit rRNA (SSU-rRNA) gene of 1429, and a large-subunit rRNA (LSU-rRNA) gene of 2698 bp. The spacer contains long direct repeats and is heterogeneous in size. The LSU-rRNA of G. muris was compared to that of the human intestinal parasite Giardia duodenalis, to the bird parasite Giardia ardeae, and to that of Escherichia coli. The LSU-rRNA has a size comparable to the 23S rRNA of E. coli but shows structural features typical for eukaryotes. Some variable regions are typically small and account for the overall smaller size of this rRNA. The structure of the G. muris LSU-rRNA is similar to that of the other Giardia rRNA, but each rRNA has characteristic features residing in a number of variable regions.

Key words

Giardia muris Protozoan parasite Ribosomal rRNA genes Ribosomal DNA sequence Large-Subunit rRNA Sequence comparison 

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Copyright information

© Springer-Verlag New York Inc 1992

Authors and Affiliations

  • Harry van Keulen
    • 1
  • Robin R. Gutell
    • 2
  • Scott R. Campbell
    • 1
  • Stanley L. Erlandsen
    • 3
  • Edward L. Jarroll
    • 1
  1. 1.Department of BiologyCleveland State UniversityClevelandUSA
  2. 2.Molecular Cellular and Developmental BiologyCampus Box 347 University of ColoradoBoulderUSA
  3. 3.Department of Cell Biology and NeuroanatomyUniversity of Minnesota Medical SchoolMinneapolisUSA

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