Biotechnology Letters

, Volume 5, Issue 4, pp 247–252 | Cite as

Determination of the volume fraction of yeast cells in a sediment or in a suspension

  • W. Borzani
  • Marina L. R. Vairo


A simple and precise method for the measurement of the volume fraction of cells in a sediment or in a suspension is described. Determinations carried out with yeast cells sediments obtained from completely fermented molasses mash lead to cells volume fraction values affected by a coefficient of variation equal to 1.9% and to a cell density equal to 1.115−1.When the method was applied to cells suspensions the coefficient of variation of the cells volume fraction was 2.9% and the cell density was 1.120−1.


Organic Chemistry Cell Suspension Cell Density Yeast Cell Cell Volume 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Aiba, S., Kitai, S., and Ishida, N. (1962). J.Gen.Appl.Microbiol., 8, 103–108.Google Scholar
  2. Aiba, S., Kitai, S., and Heima, N. (1964). J.Gen.Appl.Microbiol., 10, 243.Google Scholar
  3. Borzani, W. (1961).J.Biochem.Microbiol.Technol.Eng.,3, 235–240.Google Scholar
  4. Borzani, W., and Perego Jr., L. (1976). J. Ferment.Technol.,54, 902–906.Google Scholar
  5. Borzani, W., Gregori, R.E., and Vairo, M.L.R. (1977). Biotech. Bioeng., 14, 1363–1374.Google Scholar
  6. Haddad, S.A., and Lindegren, C.C. (1953). Appl.Microbiol., 1, 153–156.Google Scholar
  7. Koga, S., Kuribayashi, C., and Nunonura, K. (1959). J.Gen.Appl. Microbiol., 5, 35–42.Google Scholar
  8. Kubitschek, H.E. (1969). Counting and Sizing Microorganisms with the Coulter Counter. In:Methods in Microbiology, J.R. Norris and D.W. Ribbons, eds., vol. 1, pp.593–610, London, Academic Press Inc..Google Scholar
  9. Nagodawithana, T.W., and Steinkraus, K.H. (1976). Appl. Envir. Microbiol., 31, 158–162.Google Scholar
  10. Navarro, J.M., and Durand, G. (1978). Ann. Microbiol. (Inst. Pasteur), 129B, 215–224.Google Scholar
  11. Novak, M., Strehaiano, P., and Moreno, M. (1981). Biotech.Bioeng., 23, 201–211.Google Scholar
  12. Vairo, M.L.R.(1961). Influência de Diversos Fatores na Adsorção de Azul de Metileno por Leveduras Mortas. Ph.D. Thesis presen ted at the University of São Paulo, São Paulo, Brazil.Google Scholar

Copyright information

© Science and Technology Letters 1983

Authors and Affiliations

  • W. Borzani
    • 1
  • Marina L. R. Vairo
    • 2
  1. 1.Division of Chemistry and Chemical EngineeringInstituto de Pesquisas Tecnológicas do Estado de São Paulo S.A.(IPT)São PauloBrazil
  2. 2.Biochemical Engineering Laboratory, Department of Chemical Engineering, Escola PolitécnicaUniversity of São PauloSão PauloBrazil

Personalised recommendations