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Phylogenetic relationships of ferns deduced from rbcL gene sequence

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Abstract

Part of the large subunit of the ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) gene (rbcL) was sequenced from three fern species: Adiantum capillus-veneris, Botrypus strictus, and Osmunda cinnamomea var. fokiensis. This region included 1,333 base pairs, about 90% of the gene. Maximum likelihood analysis of the deduced amino acid sequences indicated that (1) Botrypus (Ophioglossaceae) clustered monophyletically with other ferns (Adiantum, Angiopteris, Osmunda); the closest relative to Botrypus among the three species was Osmunda, which did not support the hypothesis that the Ophioglossaceae are linked to the progymnosperm-seed plant lineage. (2) Eusporangiate ferns containing Botrypus (Ophioglossaceae) and Angiopteris (Marattiaceae) were a paraphyletic group. (3) Seed plants and the four fern species examined formed a monophyletic group, but ferns and bryophytes (liverwort) did not. Variations in rates of substitution for synonymous and nonsynonymous codons were found in fern lineages.

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Authors and Affiliations

  1. Botanical Gardens, University of Tokyo, 112, Hakusan, Bunkyo-ku, Tokyo, Japan

    Mitsuyasu Hasebe & Kunio Iwatsukil

  2. Makino Herbarium, 192-03, Minami-osawa, Hachiouji-shi, Tokyo, Japan

    Motomi Ito

  3. Faculty of Science, Kanazawa University, 920, Marunouchi, Kanazawa, Japan

    Rumiko Kofuji

  4. College of Integrated Arts and Sciences, University of Osaka Prefecture, 591, Mozu-ume-machi, Sakai-shi, Osaka, Japan

    Kunihiko Ueda

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  1. Mitsuyasu Hasebe
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  3. Rumiko Kofuji
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  4. Kunihiko Ueda
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  5. Kunio Iwatsukil
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Correspondence to: M. Hasebe

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Hasebe, M., Ito, M., Kofuji, R. et al. Phylogenetic relationships of ferns deduced from rbcL gene sequence. J Mol Evol 37, 476–482 (1993). https://doi.org/10.1007/BF00160428

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  • DOI: https://doi.org/10.1007/BF00160428

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