Journal of Molecular Evolution

, Volume 39, Issue 4, pp 331–339 | Cite as

Single-copy sequence homology among the GC-richest isochores of the genomes from warm-blooded vertebrates

  • Simone Cacciò
  • Paolo Perani
  • Salvatore Saccone
  • Farida Kadi
  • Giorgio Bernardi


We have hybridized a human DNA fraction corresponding to the GC-richest and gene-richest isochore family, H3, on compositional fractions of DNAs from 12 mammalian species and three avian species, representing eight and three orders, respectively. Under conditions in which repetitive sequences are competed out, the H3 isochore probe only or predominantly hybridized on the GC-richest fractions of main-band DNA from all the species investigated. These results indicate that single-copy sequences from the human H3 isochores share homology with sequences located in the compositionally corresponding compartments of the vertebrate genomes tested. These sequences are likely to be essentially formed by conserved coding sequences. The present results add to other lines of evidence indicating that isochore patterns are highly conserved in warm-blooded vertebrate genomes. Moreover, they refine recent reports (Sabeur et al., 1993; Kadi et al., 1993), and correct them in some details and also in demonstrating that the shrew genome does not exhibit the general mammalian pattern, but a special pattern.

Key words

Isochores Mammals Birds Single copy DNA sequences DNA/DNA hybridization 


  1. Aïssani B, Bernardi G (1991a) CpG islands: features and distribution in the genome of vertebrates. Gene 106:173–183Google Scholar
  2. Aïssani B, Bernardi G (1991b) CpG islands, genes and isochores in the genome of vertebrates. Gene 106:185–195Google Scholar
  3. Aïssani B, D'Onofrio G, Mouchiroud D, Gardiner K, Gautier C, Bernardi G (1991) The compositional properties of human genes. J Mol Evol 32:497–503Google Scholar
  4. Bernardi G, Olofsson B, Filipski J, Zerial M, Salinas J, Cuny G, Meunier-Rotival M, Rodier F (1985) The mosaic genome of warm-blooded vertebrates. Science 228:953–958Google Scholar
  5. Bernardi G, Mouchiroud D, Gautier C, Bernardi G (1988) Compositional patterns in vertebrate genomes: conservation and change in evolution. J Mol Evol 28:7–18Google Scholar
  6. Bernardi G (1989) The isochore organization of the human genome. Ann Rev Genet 23:637–661Google Scholar
  7. Bernardi G (1993a) The vertebrate genome: isochores and evolution. Mol Biol Evol 10:186–204Google Scholar
  8. Bernardi G (1993b) The isochore organization of the human genome and its evolutionary history: a review. Gene 135:57–66Google Scholar
  9. Carroll RL (1988) Vertebrate paleontology and evolution. Freeman, New YorkGoogle Scholar
  10. Gardiner-Garden M, Frommer M (1987) CpG islands in vertebrate genomes. J Mol Biol 196:261–282Google Scholar
  11. Jelinek WR, Toomey TP, Leinwand L, Duncan C, Biro PA, Choudary V, Weissman SM, Rubin CM, Houck CM, Deininger PL, Schmid CW (1980) Ubiquitous interspersed repeated sequences in mammalian genomes. Proc Natl Acad Sci USA 77:1398–1402Google Scholar
  12. Kadi F, Mouchiroud D, Sabeur G, Bernardi G (1994) The compositional patterns of the avian genomes and their evolutionary implications. J Mol Evol 37:544–551Google Scholar
  13. Mouchiroud D, Gautier C, Bernardi G (1988) The compositional distribution of coding sequences and DNA molecules in human and murids. J Mol Evol 27:311–320Google Scholar
  14. Mouchiroud D, D'Onofrio G, Aïssani B, Macaya G, Gautier C, Bernardi G (1991) The distribution of genes in the human genome. Gene 100:181–186Google Scholar
  15. Mouchiroud D, Bernardi G (1993) Compositional properties of coding sequences and mammalian phylogeny. J Mol Evol 37:109–116Google Scholar
  16. Novacek MJ (1992) Mammalian phylogeny: shaking the tree. Nature 356:121–125Google Scholar
  17. Nowak RM, Paradiso J (1983) Walker's Mammals of the world (4th ed), Johns Hopkins, BaltimoreGoogle Scholar
  18. Perrins CM, Middleton ALA (eds) (1985) The encyclopedia of birds. Allen & Unwin, LondonGoogle Scholar
  19. Rynditch A, Kadi F, Geryk J, Zoubak S, Svoboda J, Bernardi G (1991) The isopycnic, compartmentalized integration of Rous sarcoma virus sequences. Gene 106:165–172Google Scholar
  20. Sabeur G, Macaya G, Kadi F, Bernardi G (1993) The isochore patterns of mammalian genomes and their phylogenetic implications. J Mol Evol 37:93–108Google Scholar
  21. Saccone S, De Sario A, Della Valle G, Bernardi G (1992) The highest gene concentrations in the human genome are in T-bands of metaphase chromosomes. Proc Natl Acad Sci USA 89:4913–4917Google Scholar
  22. Saccone S, De Sario A, Wiegant J, Raap AK, Della Valle G, Bernardi G (1993) Correlations between isochores and chromosomal bands in the human genome. Proc Natl Acad Sci USA, in pressGoogle Scholar
  23. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NYGoogle Scholar
  24. Soriano P, Meunier-Rotival M, Bernardi G (1983) The distribution of interspersed repeats is nonuniform and conserved in the mouse and human genomes. Proc Natl Acad Sci USA 80:1816–1820Google Scholar
  25. Webb JE, Wallwork JA, Elgood JH (1977) Guide to living mammals. MacMillan, LondonGoogle Scholar
  26. Zerial M, Salinas J, Filipski J, Bernardi G (1986) Gene distribution and nucleotide sequence organization in the human genome. Eur J Biochem 160:479–485Google Scholar

Copyright information

© Springer-Verlag New York Inc 1994

Authors and Affiliations

  • Simone Cacciò
    • 1
  • Paolo Perani
    • 1
  • Salvatore Saccone
    • 1
  • Farida Kadi
    • 1
  • Giorgio Bernardi
    • 1
  1. 1.Laboratoire de Génétique MoléculaireInstitut Jacques MonodPlace JussieuParisFrance

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