Estimation of the concentrations of cells, astaxanthin and glucose in a culture of Phaffia rhodozyma by near infrared reflectance spectroscopy
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Near infrared reflectance spectroscopy (NIR) was employed to estimate the concentrations of cells, astaxanthin and glucose in the culture broth of Phaffia rhodozyma. The culture broth (119 samples) was directly subjected for NIR analysis without any pretreatment. When the data obtained by NIR were compared with those obtained by conventional methods, high correlation coefficients were obtained: 0.98 for cells, 0.99 for astaxanthin and 0.94 for glucose. These results suggest that NIR analysis, which is very simple and requires only 3 to 5 mm for a sample, is applicable to monitor P. rhodozyma cultures.
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- Begley, T. H., Lanza, E., Norris, K. H., and Hruschka, W. R. (1984). J. Agric. Food Chem. 32, 984–987.Google Scholar
- Iwamoto, M., Kwang, C. R., Suzuki, T., and Uozumi, J. (1984). J. Jpn. Soc. Food Sci. Technol. 31, 50–53.Google Scholar
- Iwamoto, M., Kongseree, N., Uozumi, J., and Suzuki, T. (1986a). J. Jpn. Soc. Food Sci. Technol. 33, 842–847.Google Scholar
- Iwamoto, M., Suzuki, T., Kongseree, N., Uozumi, J., and Inatsu, O. (1986b). J. Jpn. Soc. Food Sci. Technol. 33, 848–853.Google Scholar
- Norris, K. H. (1978). Proc. 6th International Cereal and Bread Congress (AACC), p.245.Google Scholar
- Okagbue, R. N. and Lewis, M. J. (1985). J. Appl. Bacteriol. 59, 243–255.Google Scholar
- Osborne, B. G. and Bearn, T. (1986). Near Infrared Spectroscopy in Food Analysis, John Willy & Sons, Inc., New York, p.36, p.86.Google Scholar
- Schroeder, W. A. and Johnson, E. A. (1995). J. Biol. Chem. 270, 18374–18379.Google Scholar
- Yano, T. and Harata, M. (1994). J. Ferment. Bioeng. 77, 659–662.Google Scholar