Biotechnology Techniques

, Volume 10, Issue 7, pp 523–528 | Cite as

A quick method for purifying bile salt-activated lipases

  • Andrew N. J. Moore
  • Helen Vakos
  • Tracey A. M. Neville
  • Peter Tonge
  • Prabhat Arya
  • Graham W. Burton
Article

Summary

A simple, one step chromatography procedure of general utility for purifying bile salt-activated lipases (BAL; cholesterol esterases) using a cholate affinity column is described. A key feature of the method is the use of a taurocholate concentration gradient in the eluent. The method has been used successfully to purify bovine and porcine BAL from commercial crude sources and BAL from human milk whey.

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Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • Andrew N. J. Moore
    • 1
  • Helen Vakos
    • 1
    • 2
  • Tracey A. M. Neville
    • 1
  • Peter Tonge
    • 1
  • Prabhat Arya
    • 1
  • Graham W. Burton
    • 1
  1. 1.Steacie Institute for Molecular Sciences, National Research Council of CanadaOttawaCanada
  2. 2.Department of ChemistryUniversity of OttawaOttawaCanada

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