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The Histochemical Journal

, Volume 25, Issue 5, pp 401–405 | Cite as

Cationic gold staining of glomerular anionic sites in archived tissue, reprocessed from paraffin wax into LR gold resin

  • N. P. Goode
  • M. Shires
  • S. R. Aparicio
  • A. M. Davison
Papers
  • 14 Downloads

Summary

Glomerular capillary wall anionic sites have been demonstrated by cationic gold staining of archived renal biopsy tissue (up to 10 years old), obtained from six patients, originally embedded in paraffin wax, and subsequently reprocessed into LR gold resin. The staining patterns at pH 2.5 and pH 7.0, demonstrating different glomerular basement membrane (GBM) anionic constituents, were compared in three patients from whom tissue directly processed into LR gold and reprocessed tissue was available. Ultrastructural preservation was poorer and shrinkage artefact greater in paraformaldehyde-lysine periodate (PLP) as opposed to formol saline-fixed reprocessed tissue. However, GBM anionic site expression was well preserved, or even enhanced (lamina rara externa, pH 7.0) in reprocessed tissue, using either fixative. Although it may not be possible to compare subtle changes in anionic site distribution in variously fixed and processed tissues, due to these artefacts, the technique enables retrospective study of charge status in archived material from disease groups in which there are distinct anionic site aberrations.

Keywords

Renal Biopsy Periodate Glomerular Basement Membrane Capillary Wall Anionic Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman & Hall 1993

Authors and Affiliations

  • N. P. Goode
    • 1
  • M. Shires
    • 1
  • S. R. Aparicio
    • 2
  • A. M. Davison
    • 3
  1. 1.Renal Research UnitSt James's University NHS TrustLeedsUK
  2. 2.Department of PathologySt James's University NHS TrustLeedsUK
  3. 3.Department of Renal MedicineSt James's University NHS TrustLeedsUK

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