Biotechnology Techniques

, Volume 9, Issue 3, pp 225–230 | Cite as

A simple method for the direct extraction of plasmid DNA from yeast

  • Michael A. Sobanski
  • J. Richard Dickinson


A rapid and simple method for the small scale isolation of shuttle plasmid DNA from Saccharomyces cerevisiae is described. It uses glass beads to break cells and reagents which are also used in bacterial mini-preps to yield plasmid DNA without chromosomal contamination in sufficient quantities to enable direct visualisation on agarose gels.


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  1. Caldwell, G.A. and Becker, J.M. (1993) Promega Notes 44, 6–9.Google Scholar
  2. Cryer, D.R., Eccleshall, R. and Marmur, J. (1975) Methods Cell. Biol. 12, 39–44.Google Scholar
  3. Ferguson, J., Groppe, J.C. and Reed, S.I. (1981) Gene 16, 191–197.Google Scholar
  4. Hoffman, C.S. and Winston, F. (1987) Gene 57, 267–272.Google Scholar
  5. Lorincz, A. (1984) Focus 6, 11Google Scholar
  6. Macnally, T., Purvis, I.J., Fothergill-Gilmore, I.A. and Brown, A.J.P. (1989) FEBS Letters 247, 312–316.Google Scholar
  7. Macreadie, I.G., Horaitis, O., Verkuylen, A.J. and Savin, K.W. (1991) Gene 104, 107–111.Google Scholar
  8. Naysmyth, K.A. and Reed, S.I. (1980) Proc. Natl. Acad. Sci. USA 77, 2119–2123.Google Scholar
  9. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular cloning: A Laboratory Manual, pp. 1.21–1.31, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.Google Scholar
  10. Sherman, F., Fink, G.R. and Hicks, J.B. (1986) Methods in yeast genetics-Laboratory Manual, pp.125–128, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.Google Scholar
  11. Ward, A.C. (1990) Nucl. Acids Res. 18, 5319.Google Scholar
  12. Ward, A.C. (1994) Promega Notes 46, 17–18.Google Scholar

Copyright information

© Chapman & Hall 1995

Authors and Affiliations

  • Michael A. Sobanski
    • 1
  • J. Richard Dickinson
    • 1
  1. 1.School of Pure and Applied Biology, University of Wales College of CardiffCardiffUK

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