Journal of Biomolecular NMR

, Volume 4, Issue 4, pp 483–490 | Cite as

Improved resolution in triple-resonance spectra by nonlinear sampling in the constant-time domain

  • Peter Schmieder
  • Alan S. Stern
  • Gerhard Wagner
  • Jeffrey C. Hoch
Research Papers


Nonlinear sampling along the constant-time dimension is applied to the constant-time HNCO spectrum of the dimerization domain of Ga14. Nonlinear sampling was used for the nitrogen dimension, while the carbon and proton dimensions were sampled linearly. A conventional ct-HNCO spectrum is compared with a nonlinearly sampled spectrum, where the gain in experiment time obtained from nonlinear sampling is used to increase the resolution in the carbonyl dimension. Nonlinearly sampled data are processed by maximum entropy reconstruction. It is shown that the nonlinearly sampled spectrum has a higher resolution, although it was recorded in less time. The constant intensity of the signal in the constant-time dimension allows for a variety of sampling schedules. A schedule of randomly distributed sampling points yields the best results. This general method can be used to significantly increase the quality of heteronuclear constant-time spectra.


Nonlinear sampling Constant-time spectra Maximum entropy reconstruction Resolution enhancement Heteronuclear triple-resonance experiments HNCO 


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Copyright information

© ESCOM Science Publishers B.V 1994

Authors and Affiliations

  • Peter Schmieder
    • 1
  • Alan S. Stern
    • 2
  • Gerhard Wagner
    • 1
  • Jeffrey C. Hoch
    • 2
  1. 1.Department of Biological Chemistry and Molecular PharmacologyHarvard Medical SchoolBostonU.S.A.
  2. 2.Rowland Institute for ScienceCambridgeU.S.A.

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