Clinical & Experimental Metastasis

, Volume 8, Issue 1, pp 27–32

Interleukin-1 increases tumor cell adhesion to endothelial cells through an RGD dependent mechanism: in vitro and in vivo studies

  • Davide Lauri
  • Maria-Cruz Bertomeu
  • F. William Orr
  • Eva Bastida
  • D. Sauder
  • Michael R. Buchanan
Article

Abstract

The effects of human recombinant interleukin-1α and β (rIL-1α; rIL-1β) on the adhesion of human A549 lung carcinoma cells and M6 melanoma cells (TC) to human endothelial cells (HECs) in vitro were studied, and on TC/lung entrapment in vivo. In vitro, there was a significant increase in TC/HEC adhesion to HECs pretreated for 4 h with rIL-1α or rIL-1β. The effects of rIL-1α and β on TC/HEC adhesion were time dependent and reached a plateau within 4–6h. TC/HEC adhesion was not blocked when measured in the presence of antibodies to either fibronectin, glycoprotein IIb/IIIa, anti-ICAM, or anti-LFA. However, enhanced TC/HEC adhesion was completely blocked in the presence of the peptide, GRGDS. In vivo, pretreatment of nude mice for 4 h with rIL-1α (given i.p. before ix. injection of TCs) enhanced TC retention in the lung 24 h later. Our data demonstrate that IL-1 enhances TC adhesion to the vascular surface both in vitro and in vivo, suggesting that IL-1 can facilitate the metastatic process.

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Copyright information

© Taylor & Francis Ltd 1990

Authors and Affiliations

  • Davide Lauri
    • 1
  • Maria-Cruz Bertomeu
    • 1
  • F. William Orr
    • 3
  • Eva Bastida
    • 2
  • D. Sauder
    • 1
  • Michael R. Buchanan
    • 1
  1. 1.Departments of Pathology and MedicineMcMaster UniversityHamiltonCanada
  2. 2.Hospital 10585ic i ProvincialBarcelonaSpain
  3. 3.Istituto Mario NegriMilanoItaly

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