European Journal of Epidemiology

, Volume 6, Issue 1, pp 29–33 | Cite as

Characterization by T1-oligonucleotide fingerprinting of three strains of human hepatitis A virus isolated in Italy

  • L. Fiore
  • E. de Paolis
  • F. Novello
  • M. Divizia
  • A. Panà
Article
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Abstract

Three human hepatitis A virus strains, all of them isolated in Italy but one acquired abroad, were analyzed by T1 - RNAase oligonucleotide mapping and by monoclonal antibody neutralization. The variation among their genomes according to T1 - maps was calculated to be about 9%, thus confirming the poor genomic variation assessed by nucleotide sequencing (1 – 10%). However T1 - maps of these Italian isolates were different from those reported in the literature (Weitz and Siegl, 1985). Neutralization by monoclonal antibody caused a reduction in titres of 2 – 2.5 log10.

This genomic stability, if confirmed, is important with a view to a valuable vaccine.

Key words

Human hepatitis A virus T1oligonucleotide fingerprinting Genomic stability 
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References

  1. 1.
    Baroudy, B.M., Ticehurst, J.R., Miele, T.A., Maizel, J.V., Purcell, R.H. and Feinstone, S.M. (1985): Sequence analysis of Hepatitis A virus c-DNA coding for capsid proteins and RNA polymerase. - Proc. Natl. Acad. Sci. USA 82: 2143–2147.Google Scholar
  2. 2.
    Cohen, J.I., Ticehurst, J.R., Purcell, R.H., Buckler-WhiteA. and Baroudy, B.M. (1987): Complete nucleotide sequence of wild-type Hepatitis A virus: comparison with different strains of Hepatitis A virus and other Picornaviruses. - J. Virol. 61, 1: 50–59.Google Scholar
  3. 3.
    DeFilippis, P., Divizia, M., Mele, A., Adamo, B. and Panà, A. (1987): Detection of Hepatitis A virus in the stools of healthy people from endemic areas. - Inter. J. Epidem. 3, 2: 172–175.Google Scholar
  4. 4.
    DeWatchers, R. and Fiers, W. (1972): Preparative two-dimensional polyacrylamide gel electrophoresis of P-labeled RNAs. - Analyt. Biochem. 49: 184–187.Google Scholar
  5. 5.
    Divizia M., Fiore L., Coulepis A.C., Mac Gregor A., Crainic R., Panà A. and Perez-Bercoff R. (1988): Monoclonal antibodies neutralize the cythopathic effect induced by the fast-growing strain of human Hepatitis A virus in “Viral Hepatitis and Liver Disease”. - Alan R. Liss. Inc. 27–30.Google Scholar
  6. 6.
    Divizia, M., Ruggeri, F., Hertschel, J., Flehming, B., PanàA. and Perez-Bercoff, R. (1986): Preliminary characterization of a fast growing strain of human hepatitis A virus. - Microbiologica 9: 269–278.Google Scholar
  7. 7.
    Domingo, E., Sabo, D., Taniguchi, T. and Weissmann, C. (1978): Nucleotide sequence heterogeneity of an RNA phage population. - Cell 13: 735–744.Google Scholar
  8. 8.
    Fiore, L., Pierangeli, A., Lombardi, F., Santoro, R., Crainic, R., Venuti, A. and Perez-Bercoff, R. (1987): Antigenic and biochemical characterization of poliovirus type 2 isolated from two cases of paralytic disease. - Intervirol. 27: 196–204.Google Scholar
  9. 9.
    Holland, J., Spindler, K., Horodyski, F., Grabau, E., Nichol, S. and Van dePol, S (1982): Rapid evolution of RNA genomes. - Science 215: 1577–1585.Google Scholar
  10. 10.
    Lemon, S.M. and Binn, L.N. (1983): Antigenic relatedness of two strains of Hepatitis A virus determined by cross-neutralization. - Inf. Imm. 42, 1: 418–420.Google Scholar
  11. 11.
    Linemeyer, D.L., Menke, J.G., Martin-Gallardo, A., Hughes, J.V., Young, A. and Mitra, S.W. (1985): Molecular cloning and partial sequencing of Hepatitis A viral c-DNA. - J. Virol. 54: 247–255.Google Scholar
  12. 12.
    Najarian, R., Caput, D., Gee, W., Potter, S.J., RenardA., Merryweather, J., Nest, G.V. and Dina, D. (1985): Primary structure and gene organization of Human Hepatitis A virus. - Proc. Natl. Acad. Sci. USA 82: 2627–2631.Google Scholar
  13. 13.
    Pasquini, P., Kahn, H.A., Pileggi, D., Panà, A., TerziJ. and D'Arca, T. (1984): Prevalence of Hepatitis A antibodies in Italy. - Inter. J. Epidemi. 13: 83–86.Google Scholar
  14. 14.
    Pedersen, F. and Haseltine, W. (1980): Analysis of the genome of an endogenous, ecotropic retrovirus of the AKR strain of mice: micromethod for detailed characterization of high molecular weight RNA. - J. Virol. 33: 349–365.Google Scholar
  15. 15.
    Venuti, A., DiRusso, C., DelGrosso, N., PattiA.M., Ruggeri, F., DeStasio, P.R., Martinello, M.G., PagnottiP., Degener, A.M., Midulla, M., Panà, A. and Perez-BercoffR. (1985): Isolation and molecular cloning of a fast growing strain of human Hepatitis A virus from its double-stranded replicative form. - J. Virol. 56, 2: 579–588.Google Scholar
  16. 16.
    Weitz, M. and Siegl, G. (1985): Variation among Hepatitis A virus strains. Genomic variation detected by T1-oligonucleotide mapping. - Virus Res. 4: 53–67.Google Scholar

Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • L. Fiore
    • 1
  • E. de Paolis
    • 1
  • F. Novello
    • 2
  • M. Divizia
    • 2
  • A. Panà
    • 2
  1. 1.Department of VirologyIstituto Superiore di SanitàRomeItaly
  2. 2.Department of Public HealthTor Vergata UniversityRomeItaly

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