Plant and Soil

, Volume 173, Issue 1, pp 11–20 | Cite as

The influence of nitrogen and potassium supply on the ammonium content of maize (Zea mays L.) leaves including a comparison of measurements made in vivo and in vitro

  • J. Gerendás
  • R. G. Ratcliffe
  • B. Sattelmacher
Research Article

Abstract

Maize seedlings were grown on either nitrate or ammonium, at two different potassium levels, and the growth analysis revealed that ammonium supply reduced shoot dry matter particularly under conditions of limited potassium supply. The ammonium content of the leaves was determined in vitro, using continuous flow analysis of plant extracts, and in vivo using 14N nuclear magnetic resonance (NMR) spectroscopy. The conventional continuous flow analysis procedure was modified by the inclusion of a gas dialysis step across a PTFE membrane and control experiments showed that this provided an effective method for avoiding the overestimation of the ammonium content of leaf tissue extracts, by eliminating interference from amino acids and amides. Excellent agreement was obtained between the non-invasive NMR method and the modified continuous flow analysis technique, and it was concluded that leaf ammonium levels are unlikely to affect growth in plants grown with an adequate potassium supply.

Key words

ammonium determination ammonium toxicity K nutrition N nutrition 14N NMR Zea mays L. 

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References

  1. Barker, A V 1968 Ammonium interactions with proteins. Biochim. Biophys. Acta 168, 447–455.Google Scholar
  2. Barker, A V, Volk, R J and Jackson, W A 1966 Root environment acidity as a regulatory factor in ammonium assimilation by the bean plant. Plant Physiol. 41, 1193–1199.Google Scholar
  3. Belton, P S, Lee, R B and Ratcliffe, R G 1985 A 14N nuclear magnetic resonance study of inorganic nitrogen metabolism in barley, maize and pea roots. J. Exp. Bot. 36, 190–210.Google Scholar
  4. Blackwell, R D, Murray, A J S and Lea, P J 1987 Inhibition of photosynthesis in barley with decreased levels of chloroplastic glutamine synthetase activity. J. Exp. Bot. 38, 1799–1809.Google Scholar
  5. Burton, D L, Gower, D A, Rutherford, P M and McGill, W B 1989 Amino acid interference with ammonium determination in soil extracts using the automated indophenol method. Commun. soil Sci. Plant Anal. 20, 555–565.Google Scholar
  6. Cramer, M D and Lewis, O A M 1993 The influence of nitrate and ammonium nutrition on the growth of wheat (Triticum aestivum) and maize (Zea mays) plants. Ann. Bot. 72, 359–365.Google Scholar
  7. Findenegg, G R 1987 A comparative study of ammonium toxicity at different constant pH of the nutrient solution. Plant and Soil 103, 239–243.Google Scholar
  8. Findenegg, G R, Nelemans, J A and Arnozis, P A 1989 Effect of external pH and Cl on the accumulation of NH4-ions in the leaves of sugar beet. J. Plant Nutr. 12, 593–601.Google Scholar
  9. Ganmore-Neumann, R and Kafkafi, U 1983 The effect of root temperature and NO3/NH4 ratio on strawberry plants. I. Growth, flowering, and root development. Agron. J. 75, 941–947.Google Scholar
  10. Gerendás J 1992 Einfluß von Form und Konzentration des Stickstoffangebotes auf Wachstum und Physiologie junger Maispflanzen (Zea mays L.). Schriftenreihe des Instituts für Pflanzenernährung und Bodenkunde, Universität Kiel, ISSN 0933-680 X, Vol 20.Google Scholar
  11. Goyal, S S and Huffaker, R C 1984 Nitrogen toxicity in plants. In Nitrogen in Crop Production. Ed. R DHauck. pp 97–118, American Society of Agronomy, Crop Science Society ofAmerica, Soil Science Society ofAmerica, Madison.Google Scholar
  12. Harada, T, Takaki, H and Yamada, Y 1968 Effect of nitrogen sources on the chemical components of young plants. Soil Sci. Plant Anal. 14, 47–55.Google Scholar
  13. Jungk, A 1977 Wirkung von Ammonium- und Nitratstickstoff auf das Wachstum und die Zusammensetzung von Pflanzen. Landw. Forsch. Sonderheft 34 II, 18–26.Google Scholar
  14. Kendall, A C, Wallsgrove, R M, Hall, N P, Turner, J C and Lea, P J 1986 Carbon and nitrogen metabolism in barley (Hordeum vulgare L.) mutants lacking ferredoxin-dependent glutamate synthase. Planta 168, 316–323.Google Scholar
  15. Krogmann, D W, Jagendorf, A T and Avron, M 1959 Uncouplers of spinach chloroplast photosynthetic phosphorylation. Plant Physiol. 34, 272–277.Google Scholar
  16. Lee, R B and Ratcliffe, R G 1983 Development of an aeration system for use in plant tissue NMR experiments. J. Exp. Bot. 34, 1213–1221.Google Scholar
  17. Lee, R B and Ratcliffe, R G 1991 Observations on the subcellular distribution of the ammonium ion in maize root tissue using in-vivo 14N-nuclear magnetic resonance spectroscopy. Planta 183, 359–367.Google Scholar
  18. Lips, S H, Leidi, E O, Liberbush, M, Soares, M I M and Lewis, O E M 1990 Physiological aspects of ammonium and nitrate fertilization. J. Plant Nutr. 13, 1271–1289.Google Scholar
  19. Magalhaes, J R and Huber, D M 1989 Ammonium assimilation in different plant species as affected by nitrogen form and pH control in solution culture. Fert. Res. 21, 1–6.Google Scholar
  20. Magalhaes, J S and Wilcox, G E 1983 Tomato growth and nutrient uptake patterns as influenced by nitrogen form and light intensity. J. Plant Nutr. 6, 941–956.Google Scholar
  21. Martin, J-B, Bligny, R, Rébeillé, F, Douce, R, Leguay, J-J, Mathieu, Y and Guern, J 1982 A 31P nuclear magnetic resonance study of intracellular pH of plant cells cultivated in liquid medium. Plant Physiol. 70, 1156–1161.Google Scholar
  22. Maynard, D N, Barker, A V and Lachman, W H 1968 Influence of potassium on the utilization of ammonium by tomato plants. Proc. Am. Soc. Hort. Sci. 92, 537–542.Google Scholar
  23. Mills, H A and Jones, J B 1979 Nutrient deficiencies and toxicities in plants: Nitrogen. J. Plant Nutr. 1, 101–122.Google Scholar
  24. Novozamsky, I, Houba, V J G, vanEck, R and vanVark, W 1983 A novel digestion technique for multi-element plant analysis. Commun. Soil Sci. Plant Anal. 14, 239–248.Google Scholar
  25. Patton, C J and Crouch, S R 1977 Spectrophotometric and kinetics investigation of the Berthelot reaction for the determination of ammonia. Anal. Chem. 49, 464–469.Google Scholar
  26. Puritch, G S and Barker, A V 1967 Structure and function of tomoto leaf chloroplasts during ammonium toxicity. Plant Physiol. 42, 1229–1238.Google Scholar
  27. SAS/STAT 1988 SAS Institute Inc. SAS/STAT User's Guide, Release 6.03 Edition, Cary, NC 27512–8000, USA: SAS Institute Inc., 1988, 1028 pp.Google Scholar
  28. Schrader, L E, Domska, D, Jung, P E and Peterson, L A 1972 Uptake and assimilation of ammonium-N and nitrate-N and the influence on the growth of corn (Zea mays L.). Agron. J. 64, 690–695.Google Scholar
  29. Walker, K A, Keys, A J and Givan, C V 1984 Effect of L-methionine sulphoximine on the products of photosynthesis in wheat (Triticum aestivum) leaves. J. Exp. Bot. 35, 1800–1810.Google Scholar
  30. Wilcox, G E, Magalhaes, J R and Silva, F L I M 1985 Ammonium and nitrate concentration as factors in tomato growth and nutrient uptake. J. Plant Nutr. 8, 989–998.Google Scholar
  31. Zornoza, P, Caselles, J and Carpena, O 1989 Effect of NO3: NH4 ratio and light intensity on nitrogen partitioning in pepper plants. J. Plant Nutr. 12, 307–316.Google Scholar

Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • J. Gerendás
    • 1
  • R. G. Ratcliffe
    • 2
  • B. Sattelmacher
    • 1
  1. 1.Institute for Plant Nutrition and Soil ScienceUniversity KielKielGermany
  2. 2.Department of Plant SciencesUniversity of OxfordOxfordUK

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