Biotechnology Techniques

, Volume 1, Issue 4, pp 245–250

A novel immobilized hybridoma reactor for the production of monoclonal antibodies

  • O. T. Ramirez
  • R. Mutharasan
  • W. E. Magee
Culture systems

DOI: 10.1007/BF00155463

Cite this article as:
Ramirez, O.T., Mutharasan, R. & Magee, W.E. Biotechnol Tech (1987) 1: 245. doi:10.1007/BF00155463

Summary

Mouse hybridoma cells were succesfully cultivated for more than 640 hours in the interparticle spaces of a tubular reactor packed with spherical glass beads. The maximum monoclonal antibody (MAb) concentration attained was 110 mg/l and a viable cell density in the order of 1 × 107 cells/ml was achieved. A productivity per reactor void volume of 5.2 mg MAb/hr/l was obtained, which is comparable to the best systems currently in use.

Copyright information

© Chapman & Hall 1987

Authors and Affiliations

  • O. T. Ramirez
    • 1
  • R. Mutharasan
    • 1
  • W. E. Magee
    • 2
  1. 1.Department of Chemical EngineeringDrexel UniversityPhiladelphiaUSA
  2. 2.Department of Bioscience and BiotechnologyDrexel UniversityPhiladelphiaUSA

Personalised recommendations