A novel immobilized hybridoma reactor for the production of monoclonal antibodies
- Cite this article as:
- Ramirez, O.T., Mutharasan, R. & Magee, W.E. Biotechnol Tech (1987) 1: 245. doi:10.1007/BF00155463
- 13 Downloads
Mouse hybridoma cells were succesfully cultivated for more than 640 hours in the interparticle spaces of a tubular reactor packed with spherical glass beads. The maximum monoclonal antibody (MAb) concentration attained was 110 mg/l and a viable cell density in the order of 1 × 107 cells/ml was achieved. A productivity per reactor void volume of 5.2 mg MAb/hr/l was obtained, which is comparable to the best systems currently in use.