Biotechnology Techniques

, Volume 1, Issue 4, pp 245–250 | Cite as

A novel immobilized hybridoma reactor for the production of monoclonal antibodies

  • O. T. Ramirez
  • R. Mutharasan
  • W. E. Magee
Culture systems


Mouse hybridoma cells were succesfully cultivated for more than 640 hours in the interparticle spaces of a tubular reactor packed with spherical glass beads. The maximum monoclonal antibody (MAb) concentration attained was 110 mg/l and a viable cell density in the order of 1 × 107 cells/ml was achieved. A productivity per reactor void volume of 5.2 mg MAb/hr/l was obtained, which is comparable to the best systems currently in use.


Monoclonal Antibody Viable Cell Cell Density Glass Bead Void Volume 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Chapman & Hall 1987

Authors and Affiliations

  • O. T. Ramirez
    • 1
  • R. Mutharasan
    • 1
  • W. E. Magee
    • 2
  1. 1.Department of Chemical EngineeringDrexel UniversityPhiladelphiaUSA
  2. 2.Department of Bioscience and BiotechnologyDrexel UniversityPhiladelphiaUSA

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