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Biotechnology Techniques

, Volume 10, Issue 11, pp 809–814 | Cite as

Characteristics of immobilized Rhizopus oryzae in polyurethane foam cubes

  • Yan Sun
  • Y. L. Li
  • H. Yang
  • S. Bai
  • Z. D. Hu
Article

Summary

Rhizopus oryzae was immobilized in polyurethane foam cubes. The effects of the cube size on cell immobilization, cell growth and L(+)-lactic acid production were studied. By the natural attachment method, R. oryzae could be easily immobilized in the polyurethane foam cubes larger than 2.5 × 5 × 5 mm3. The use of small cubes for R. oryzae immobilization was very effective to increase the productivity of L(+)-lactic acid by the immobilized cells. Although it was difficult for smaller cubes to be completely full of the mycelia, increasing the inoculum size in immobilizations was effective to increase the immobilization ratio (a ratio of the number of the cubes containing cells to the total number of cubes).

Keywords

Foam Cell Growth Immobilization Lactic Acid Polyurethane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Dong, X.-Y., Bai, S. and Sun, Y. (1996). Biotechnol. Lett. 18: 225–228Google Scholar
  2. Sun, Y., Shi, Q.H., Bai, S. and Li, Y.L. (1996). In: Proc. 5th World Congress of Chem. Eng., vol. 2, pp. 330–335, San Diego, CA.Google Scholar

Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • Yan Sun
    • 1
  • Y. L. Li
    • 1
  • H. Yang
    • 1
  • S. Bai
    • 1
  • Z. D. Hu
    • 1
  1. 1.Department of Chem. Eng. and Research Centre for BiotechnologyTianjin UniversityTianjinP. R. China

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