Biotechnology Techniques

, Volume 9, Issue 1, pp 45–48 | Cite as

Stability of arginase in reverse micelles

  • S. Subramani
  • D. Madamwar
Article

Summary

The influence of glycerol and sodium chloride on the stability of arginase entrapped in reverse micellar system of non-ionic surfactant Triton X - 100/n-hexanol/n-hexane, was studied. Entrapped enzyme shows better stability, further enhanced by the addition of these additives. However, the stability with these additives was found to be much better in reverse micelles.

Keywords

Enzyme Sodium Chloride Surfactant Glycerol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Boicelli, C.A. Conti, F. Giomini, M. and Giuliani, A.M. (1985) in Physical Methods on Biological Membranes and Their Model Systems (Conti, F. Blumberg, W. E. deGier, J. and Pocchiari, F., eds.) p. 141, Plenum Press, New York.Google Scholar
  2. Grandi, C. Smith, R. E. and Luisi, P. L. (1980) J. Biol. Chem. 256(2), 837 - 843.Google Scholar
  3. Luisi, P. L. Giomini, M. Pileni, M. P. and Robinson, B. H. (1988) Biochimica et Biophysica Acta, 947, 209 - 246.Google Scholar
  4. Luthi, P. and Luisi, P. L. (1984) J. Am. Chem. Soc. 106, 7285 - 7286.Google Scholar
  5. Martinek, K. Levashov, A. V. Pantin, V. I. and Berezin, I. V. (1978) Dokl. Akad. Nauk, SSSR 238, 626 - 629.Google Scholar
  6. Prazeres, D. M. F. Garcia, F. A. P. and Cabral, J. M. S. (1992) J. Chem. Tech. Biotechnol. 53, 159 - 64.Google Scholar
  7. Ruckenstein, E. and Karpe, P. (1990) Biotech. Lett. 12(4), 241 - 246.Google Scholar
  8. Serralheriro, M. L. Empis, J. M. and Cabral. (1990) Biotech. Lett. 12(3), 167 - 172.Google Scholar
  9. Subramani, S. and Madamwar, D. [a] Enzyme. Microb. Technol. - To appear.Google Scholar
  10. Subramani, S. and Madamwar, D. [b] Biotech. Bioeng. - Submitted.Google Scholar
  11. Varely, H. (1976) in Practical Clinical Biochemistry, Arnold- Heinemann publishers (India) Pvt. Ltd., New Delhi, 161 -162.Google Scholar

Copyright information

© Chapman & Hall 1995

Authors and Affiliations

  • S. Subramani
    • 1
  • D. Madamwar
    • 1
  1. 1.Post-Graduate Department of BiosciencesSardar Patel University Vallabh VidyanagarGujaratIndia

Personalised recommendations