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A rapid and simple screening technique for potential crude oil degrading microorganisms

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Biotechnology Techniques

Summary

A rapid and simple technique has been developed for screening potential crude oil degrading bacteria using the redox indicator 2,6-dichlorophenol indophenol in Bushnell and Haas medium with crude oil and a microtitre plate. Bacteria possessing high crude oil degrading potential turn the medium colourless after 24 h incubation but, depending upon the time taken for the change in colour, relative abilities of different cultures can be ascertained.

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Authors and Affiliations

  1. Department of Microbiology & Biotechnology Centre, Faculty of Science, M.S.University of Baroda, 390 002, Baroda, India

    Anjana J. Desai

  2. Environmental Science & Applied Biology Division, Research Centre, Indian Petrochem. Corpn. Ltd., 391 346, Baroda, India

    Jitendra D. Desai

Authors
  1. K. G. Hanson
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  2. Jitendra D. Desai
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  3. Anjana J. Desai
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Hanson, K.G., Desai, J.D. & Desai, A.J. A rapid and simple screening technique for potential crude oil degrading microorganisms. Biotechnol Tech 7, 745–748 (1993). https://doi.org/10.1007/BF00152624

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  • DOI: https://doi.org/10.1007/BF00152624

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