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Biotechnology Techniques

, Volume 7, Issue 10, pp 723–726 | Cite as

Agar, an alternative to agarose in analytical gel electrophoresis

  • C. D. Viljoen
  • B. D. Wingfield
  • M. J. Wingfield
Article

Summary

Agarose is purified from the polysaccharide complex, agar. This purified fraction forms a neutral gel matrix that is commonly used in gel electrophoresis. We have found that for many routine uses, agar which is considerably less expensive than agarose can be used with satisfactory results.

Keywords

Agar Agarose Polysaccharide Electrophoresis Satisfactory Result 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Aaij, C., and Borst, P. (1972) Biochim. Biophys. Acta 269, 192–200.Google Scholar
  2. Araki, C. (1937) J. Chem. Soc. Japan 58, 1338–1350.Google Scholar
  3. Araki C., and Arai K. (1956) Bull. Chem. Soc. Japan 29, 339–345.Google Scholar
  4. Hayward, G. S., and Smith, M. G. (1972) J. Mol. Biol. 63, 383–395.Google Scholar
  5. Helling, R. B., Goodman, H. M., and Boyer, H. W. (1974) J. Virol. 14, 1235–1244.Google Scholar
  6. Maniatis, T., Fritsch, E. F., and Sambrook, J. (1989) Molecular Cloning. A laboratory manual. 2nd Edition, Cold Spring Harbor Laboratory, New York.Google Scholar
  7. McIndoe, W., and Munro, H. N. (1967) Biochim. Biophys. Acta 134, 458–460.Google Scholar
  8. Morrice, L. M., McLean, M. W., Long, W. F., and Williamson, F. B. (1983) Eur. J. Biochem. 133 673–684.Google Scholar
  9. Santos G. A., and Doty M. S. (1983) Botanica Marina 26, 31–34.Google Scholar
  10. Serwer P. (1983) Electrophoresis 4, 375–382.Google Scholar
  11. Sharp, P. A., Sugden, B., and Sambrook, J. (1973) Biochemistry 12, 3055–3063.Google Scholar
  12. Williams, B.L., and Wilson, K. (1979) Principles and Techniques of Practical Biochemistry. 2nd Edition, Edward Arnold Ltd., London.Google Scholar

Copyright information

© Chapman & Hall 1993

Authors and Affiliations

  • C. D. Viljoen
    • 1
  • B. D. Wingfield
    • 1
  • M. J. Wingfield
    • 1
  1. 1.Department of Microbiology and BiochemistryUniversity of the Orange Free StateBloemfonteinSouth Africa

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