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Biotechnology Techniques

, Volume 9, Issue 8, pp 583–588 | Cite as

Adsorption equilibrium and dynamics of lactase/CM-Sephadex system

  • Şebnem Harsa
  • Yekta Göksungur
  • Ulgar Güvenç
Article
  • 24 Downloads

Summary

Partitioning behaviour and adsorption isotherms of lactase/CM-Sephadex system at equilibrium were investigated together with the adsorption kinetics in this study. Maximum adsorption was obtained at the pH values between 5.5–6.0. Adsorption isotherm was a close fit to the Langmuir model.

Keywords

Adsorption Isotherm Adsorption Equilibrium Adsorption Kinetic Langmuir Model Partitioning Behaviour 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Nomenclature

a

specific mass transfer area

Dm

molecular diffusion coefficient (m2/sec)

e1, e2

charge of the protein and the gel

k

apparent mass transfer coefficient (s-1)

ka

global mass transfer coefficient

f

partition coefficient

Kp

dissociation constant for adsorbent-adsorbate complex, (mg/mL solvent)

p

equilibrium concentration of free enzyme, (mg free enzyme/mL solution)

q

equilibrium concentration of adsorbed enzyme, (mg ads./mL gel)

qm

maximum adsorption capacity, (mg ads./ml gel)

Re

particle Reynolds number

Sh

Sherwood number

Vg/V

gel volume (mL)/bulk solvent volume (mL)

Z

dimensionless extent of adsorption

α

Kp/Po , model parameter

α′

(α/β) +1 , model parameter

β

Vg qm / V Po , model parameter

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References

  1. Harsa, S., Pyle, D.L. & Zaror, C.A. (1990). In: Separations for Biotechnology 2, (Ed. D.L. Pyle). Elsevier Applied Science pp 658.Google Scholar
  2. Harsa, S., Zaror, C.A. & Pyle, D.L. (1993). Enzyme and Microbial Techn. 15: 906–915.Google Scholar

Copyright information

© Chapman & Hall 1995

Authors and Affiliations

  • Şebnem Harsa
    • 1
  • Yekta Göksungur
    • 2
  • Ulgar Güvenç
    • 2
  1. 1.Izmir Institute of TechnologyBasmaneTurkey
  2. 2.Dept. of Food Eng., Faculty of EngineeringEge UniversityBornovaTurkey

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