Development of an electro-transformation system for Escherichia coli DH10B
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Summary
Escherichia coli can be transformed to high efficiencies by subjecting a mixture of cells and DNA to a brief but intense electrical field. Factors that affect the transformation efficiency of E.coli strain DH10B were analysed. Optimal conditions gave an efficiency of 108 to 109 transformants/μg DNA with E.coli strains K803 and DH10B, and plasmids pB1221.23 and pBSK+. The use of ligated DNA resulted in 106 transformants/μg DNA. Detailed protocols for these systems are given.
Keywords
Optimal Condition Transformation Efficiency Detailed Protocol Intense Electrical Field Strain DH10B
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© Science & Technology Letters 1993