Plant and Soil

, Volume 160, Issue 1, pp 11–20 | Cite as

Biomass allocation, phosphorus nutrition and vesicular-arbuscular mycorrhizal infection in clones of Yorkshire Fog, Holcus lanatus L. (Poaceae) that differ in their phosphate uptake kinetics and tolerance to arsenate

  • A. A. Meharg
  • J. Bailey
  • K. Breadmore
  • M. R. Macnair
Research Articles

Abstract

Biomass and phosphorus allocation were determined in arsenate tolerant and non-tolerant clones of the grass Holcus lanatus L. in both solution culture and in soil. Arsenate is a phosphate analogue and is taken up by the phosphate uptake system. Tolerance to arsenate in this grass is achieved by suppression of arsenate (and phosphate) influx. When clones differing in their arsenate tolerance were grown in solution culture with a range of phosphate levels, a tolerant clone did not fare as well as a non-tolerant at low levels of phosphate nutrition in that it had reduced shoot biomass production, increased biomass allocation to the roots and lower shoot phosphorus concentration. At a higher level of phosphate nutrition there was little or no difference in these parameters, suggesting that differences at lower levels of phosphate nutrition were due solely to differences in the rates of phosphate accumulation. In experiments in sterile soil (potting compost) the situation was more complicated with tolerant plants having lower growth rates but higher phosphorus concentrations.

The gene for arsenate tolerance is polymorphic in arsenate uncontaminated populations. When phosphorus concentration of tolerant phenotypes was determined in one such population, again tolerants had a higher phosphorus status than non-tolerants. Tolerants also had higher rates of vesicular-arbuscular mycorrhizal (VAM) infection. The ecological implications of these results are that it appears that suppression of the high affinity uptake system, is at least in part, compensated by increased mycorrhizal infection.

Key words

arsenic biomass allocation metal tolerance phosphorus nutrition 

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References

  1. Allen, S E 1974 Chemical analysis of Ecological Materials, Blackwell, Oxford.Google Scholar
  2. Bieleski, R L 1973 Phosphate pools, phosphate transport, and phosphate availability. Annu. Rev. Plant. Physiol. 24, 225–252.Google Scholar
  3. Bolan, N S 1991 A critical review of mycorrhizal fungi in the uptake of phosphorus by plants. Plant and Soil 134, 189–207.Google Scholar
  4. Chapin, F S 1980 The mineral nutrition of wild plants. Annu. Rev. Ecol. Syst. 11, 233–260.Google Scholar
  5. Chapin, F S 1983 Adaptation of selected trees and grasses to low availability of phosphorus. Plant and Soil 72, 283–287.Google Scholar
  6. Chapin, F S and Bieleski, R L 1982 Mild phosphorus stress in barley and a related low-phosphorus-adapted barleygrass: Phosphorus fractions and phosphate absorption in relation to growth. Physiol. Plant. 54, 309–317.Google Scholar
  7. Chapin, F S, Follett, J M and O'Connor, K F 1982 Growth, phosphate absorption, and phosphorus chemical fractions in two Chionochloa species. J. Ecol. 70, 305–321.Google Scholar
  8. Clarkson, D T and Lüttge, U 1991 Mineral nutrition: Inducible and repressible nutrient transport systems. Prog. Bot. 52, 93–112.Google Scholar
  9. Cress, W A, Throneberry, G O and Lindsay, D L 1979 Kinetics of phosphorus absorption by mycorrhizal and nonmycorrhizal tomato roots. Plant Physiol. 64, 484–487.Google Scholar
  10. Lefebvre, D and Glass, D M 1982 Regulation of phosphate influx in barley roots. Effects of phosphate deprivation and reduction of influx on provision with orthophosphate. Physiol. Plant. 54, 199–206.Google Scholar
  11. Macnair, M R, Cumbes, Q J and Meharg, A A 1992 The genetics of arsenate tolerance in Yorkshire Fog, Holcus lanatus L. Heredity 69, 325–335.Google Scholar
  12. Meharg, A A, Cumbes, Q J and Macnair, M R 1992 Preadaptation of Yorkshire Fog, Holcus lanatus L. (Poaceae) to arsenate tolerance. Evolution 47, 313–316.Google Scholar
  13. Meharg, A A and Macnair, M R 1990 An altered phosphate uptake system in arsenate tolerant Holcus lanatus. New Phytol. 116, 29–35.Google Scholar
  14. Meharg, A A and Macnair, M R 1991 Uptake, accumulation and translocation in arsenate tolerant and non-tolerant Holcus lanatus L. New Phytol. 117, 225–231.Google Scholar
  15. Meharg, A A and Macnair, M R 1992a Suppression of the high affinity phosphate uptake system: a mechanism of arsenate tolerance in Holcus lanatus L. J. Exp. Bot. 43, 519–524.Google Scholar
  16. Meharg, A A and Macnair, M R 1992b Genetic correlation between arsenate tolerance and the rate of influx of arsenate and phosphate in Holcus lanatus L. Heredity 69, 336–341.Google Scholar
  17. Meharg, A A and Macnari, M R 1992c Polymorphism and physiology of arsenate tolerance in Holcus lanatus L. from an uncontaminated site. Plant and Soil 146, 219–225.Google Scholar
  18. Nye, P H 1977 The rate limiting step in plant-nutrient absorption from soil. Soil. Sci. 123, 292–297.Google Scholar
  19. Phillips, J M and Hayman, D S 1970 Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Trans. Brit. Mycol. Soc. 55, 158–162.Google Scholar
  20. Rorison, I H 1968 The response to phosphorus of some ecologically distinct plant species. 1. Growth rates and phosphorus absorption. New Phytol. 67, 913–923.Google Scholar
  21. Silberbush, M and Barker, S A 1983 Sensitivity of simulated phosphorus uptake to parameters used by a mechanistic mathematical model. Plant and Soil 74, 93–100.Google Scholar
  22. Snaydon, R W and Bradshaw, A D 1962 Differences between natural populations of Trifolium repens L. in response to mineral nutrients. 1. Phosphate. J. Exp. Bot. 13, 422–434.Google Scholar
  23. Snellgrove, R C, Slittoesser, W E, Stribley, D P and Tinker, P B 1982 The distribution of carbon and the reward of the fungal symbiant in leek plants with vesicular-arbuscular mycorrhizae. New Phytol. 92, 75–87.Google Scholar

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • A. A. Meharg
    • 1
  • J. Bailey
    • 1
  • K. Breadmore
    • 1
  • M. R. Macnair
    • 1
  1. 1.Department of Biological Sciences, Hatherly LaboratoriesUniversity of ExeterExeterEngland
  2. 2.Institute for Terrestrial Ecology, Monks Wood, Abbotts RiptonHuntingdonUK

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