, Volume 4, Issue 1, pp 13–28

Electron microscopy of hybridoma cells with special regard to monoclonal antibody production

  • M. Al-Rubeai
  • D. Mills
  • A. N. Emery
Original Research Papers


Electron microscopy of mouse hybridoma cell lines shows that the major difference between non, low and high producer cell lines is the amount of endoplasmic reticulum. Vesicular-tubular or cavernous structures of endoplasmic reticulum, which can survive long after cell death, are particularly abundant in producer cell lines. Immunogold labelling with anti-mouse IgG reveals that antibodies are predominantly located in these structures. The cell membrane undergoes structural changes during the late stages of batch culture with the disappearance of microvilli and the appearance of blebs and deep indentations. Necrosis disrupts the cytoplasmic structures and the nucleus is last to degrade.

Key words

monoclonal antibody hybridoma electron microscopy endoplasmic reticulum 


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Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • M. Al-Rubeai
    • 1
  • D. Mills
    • 2
  • A. N. Emery
    • 1
  1. 1.School of Chemical EngineeringUniversity of BirminghamBirminghamUK
  2. 2.School of BiochemistryUniversity of BirminghamBirminghamUK

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