Cytotechnology

, Volume 1, Issue 2, pp 159–169

Egg yolk lipoprotein, a new supplement for the growth of mammalian cells in serum-free medium

Egg yolk lipoprotein for serum-free growth of cells
  • Hiroki Murakami
  • Yuichi Okazaki
  • Koji Yamada
  • Hirohisa Omura
Article

Abstract

Egg yolk lipoprotein promoted growth of a wide variety of mammalian cell lines, including plasma-cytomas and epithelial cell lines, in serum-free medium. The lipoprotein was active for cell growth when used with insulin, transferrin, ethanolamine and selenite. The most active lipoprotein fraction (YLP-pI7.5) was purified to give a single peak by chromatofocusing and gel filtration, and was homogeneous on a 0.35% agarose gel electrophoretogram. The lipoprotein was characterised as a very low density lipoprotein with a protein content of only 1.3%. This lipoprotein had an optimal concentration of 300 μg/ml (4 μg protein/ml). It was easily separable from proteinous molecules secreted into the serum-free medium by the cells, since it floated on the surface of the medium after addition of ammonium sulfate, to precipitate protein, and centrifugation. An associated structure of lipid and protein seemed to be still necessary for the lipoprotein to exhibit a growth promoting activity.

Key words

lipoprotein egg yolk serum-free medium cell growth monoclonal antibody ITES 

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Copyright information

© Martinus Nijhoff Publishers 1988

Authors and Affiliations

  • Hiroki Murakami
    • 1
  • Yuichi Okazaki
    • 1
  • Koji Yamada
    • 1
  • Hirohisa Omura
    • 1
  1. 1.Institute of Food Science and Technology, 46-09, Department of AgricultureKyushu UniversityFukuokaJapan

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