Photosynthesis Research

, Volume 38, Issue 2, pp 153–157 | Cite as

Fluorescence studies on interaction between phospho-LHC II and subchloroplast Photosystem 1 preparations

  • Svetlana M. Kochubey
  • Victor V. Shevchenko
  • Olga I. Volovik
Research Papers
Received:
Accepted:

Abstract

Chloroplast proteins were phosphorylated under two test conditions: ‘white light’ irradiance alone and ‘white light’ irradiance with the addition of glucose and glucose oxidase, used to produce an anaerobic medium. The interaction of phospho-LHC II with Photosystem 1 (PS 1) was studied for two types of PS I preparation. Changes in the chlorophyll a/b ratio and the ratio of 650 and 680 nm band intensities (E650/E680) in fluorescence excitation spectra were used in calculating the phospho-LHC II portion which became associated with PS 1. It is shown that the associated portion of phospho-LHC II varies for each of the PS 1 preparations and phosphorylation procedures. Possible conclusions as regards the transfer of various sets of LHC II subpopulations under different phosphorylation procedures and the differences of interaction with PS 1 are discussed.

Key words

light-harvesting chlorophyll a/b protein complex II phosphorylation of chloroplast membrane proteins low temperature fluorescence spectra fluorescence excitation spectra 

Abbreviations

PS 1

Photosystem 1

PS 2

Photosystem 2

LHC II

light-harvesting chlorophyll a/b protein complex II

Chl

chlorophyll

ϕ

fluorescence quantum yield

τf

life time of fluorescence at λ=685 nm

F735

fluorescence band with a maximum at 735 nm

F685

fluorescence band with a maximum at 685 nm

E650/E680

ratio of amplitudes in excitation fluorescence spectrum at 650 and 680 nm

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References

  1. Allen JF (1992) Protein phosphorylation in regulation of photosynthesis. Biochim Biophys Acta 1098: 275–335Google Scholar
  2. Allen JF and Melis A (1988) The rate of P-700 photooxidation under continuous illumination is independent of State 1-State 2 transitions in the green alga Scenedesmus obliguus. Biochim Biophys Acta 933: 95–106Google Scholar
  3. Anderson JM, Waldron JC and Thorne SW (1987) Chlorophyll-protein complexes of spinach and barley thylakoids. Spectral characterization of six complexes resolved by improved electrophoretic procedure. FEBS Lett 92: 227–233Google Scholar
  4. Bassi K and Simpson D (1987) Chlorophyll-protein complexes of barley Photosystem I. Eur J Biochem 163: 221–230Google Scholar
  5. Bennett J (1991) Protein phosphorylation in green plant chloroplasts. Annu Rev Plant Physiol, Plant Mol Biol 42: 281–311Google Scholar
  6. Delepelaire P and Wollman F-A (1985) Correlation between fluorescence and phosphorylation changes in thylakoid membranes of Chlamydomonas reinhardtii in vivo: A kinetic analysis. Biochim Biophys Acta 809: 277–283Google Scholar
  7. Forti G and Vianelli A (1988) Influence of thylakoid protein phosphorylation on Photosystem 1 photochemistry. FEBS Lett 231: 95–98Google Scholar
  8. Harrison MA and Allen JF (1992) Protein phosphorylation and the Mg2+ influence light-harvesting and electron transport in chloroplast thylakoid membrane material containing only chlorophyll-a/b-binding light-harvesting complex of Photosystem II and Photosystem I. Eur J Biochem 204: 1107–1114Google Scholar
  9. Haworth P and Melis A (1983) Phosphorylation of chloroplast thylakoid membrane proteins does not increase the absorption cross-section of Photosystem 1. FEBS Lett 160: 277–280Google Scholar
  10. Hodges M and Moya I (1988) Time-resolved chlorophyll fluorescence studies on pigment-protein complexes from photosynthetic membranes. Biochim Biophys Acta 935: 41–52Google Scholar
  11. Holzwarth AK (1986) Fluorescence lifetimes in photosynthetic systems. Photochem Photobiol 43: 707–725Google Scholar
  12. Kochubey SM, Samokhval EG, Klimusheva GV and Delukov AA (1980) Temperature dependence of the absolute fluorescence yields of chloroplast fragments. Arch Biochem Biophys 200: 65–71Google Scholar
  13. Kochubey SM, Volovik OI, Juravel TT, Ruban AV and Samokhval EG (1988) Lateralnyi perenos svetosobirayuschego kompleksa fotosystemy 2 pri fosforilirovanii ego belkovoi chasti. (Lateral migration of light-harvesting complex of Photosystem 2 under phosphorylation of its protein part). Fiziol Rast 35: 5–13Google Scholar
  14. Kyle DJ, Kuang TY, Watson JL and Arntzen CJ (1984) Movement of sub-population of the light-harvesting complex (LHC II) from grana to stroma lamellae as a consequence of its phosphorylation. Biochim Biophys Acta 765: 89–96Google Scholar
  15. Larsson UK, Örgen E, Öquist G and Andersson B (1986) Electron transport and fluorescence studies on the functional interaction between phospho-LHC II and Photosystem I in isolated stroma lamellae vesicles. Photobiochem Photobiophys 13: 29–39Google Scholar
  16. Larsson UK, Sundby S and Andersson B (1987) Characterization of two different subpopulations of spinach light-harvesting chlorophyll a/b-protein complex (LHC II): Polypeptide composition, phosphoryaltion pattern and association with Photosystem II. Biochim Biophys Acta 894: 59–68Google Scholar
  17. Ruban AV and Kochubey SM (1989) Changes in Photosystem 1 characteristics induced by phosphorylation of chloroplast proteins in plants grown under various environmental conditions. 2. Emission and excitation spectra. Photosynthetica 23: 173–180.Google Scholar
  18. Svensson P, Andreasson E and Albertsson PA (1991) Heterogeniety among Photosystem 1. Biochim Biophys Acta 1060: 45–50Google Scholar
  19. Vernon LP (1960) Spectrophotometric determination of chlorophylls and pheophytins in plant extracts. Anal Chem 32: 1144–1150Google Scholar
  20. Volovik OI and Kochubey SM (1989) Changes in Photosystem 1 characteristics induced by phosphorylation of chloroplast proteins in plant grown under various irradiances. 1. Migration and incorporation of light-harvesting chlorophyll-a/b protein. Photosynthetica 23: 36–42Google Scholar
  21. Volovik OI, Ruban AV and Kochubey SM (1991) Perenos kompleksov fotosistemy 2 v mezhgrannyje tilakoidy pri fosforilirovanii membrannykh belkov khloroplastov (Transfer of Photosystem II complexes to integrana thylakoids under membrane protein phosphorylation). Dokl Akad Nauk SSSR 319: 763–767Google Scholar

Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Svetlana M. Kochubey
    • 1
  • Victor V. Shevchenko
    • 1
  • Olga I. Volovik
    • 1
  1. 1.Institute of Plant Physiology and GeneticsUkrainian Academy of SciencesKievUkraine

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