European Journal of Epidemiology

, Volume 4, Issue 4, pp 419–425 | Cite as

Recent advances in the development of a Streptococcus mutans vaccine

  • P. Klein
  • M. Scholler

Key words

Streptococcus mutans Vaccine Dental caries 


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  1. 1.
    Ackermans F., Klein J.P., Ogier J., Bazin H., Cormont F and Frank R.M. (1985): Purification and characterization of a saliva interacting cell surface protein from Streptococcus mutans serotype f using monoclonal antibody immunoaffnity chromatography. Biochem J., 228: 211–217.Google Scholar
  2. 2.
    Ayakawa G. Y., Siegel J.L., Crowley P. T.and Bleiweis A.S. (1985): Immunochemical studies of Streptococcus mutans BHT cell membrane: detection of determinants cross reactive with human heart tissue. Infect. Immun., 48: 2880–2886Google Scholar
  3. 3.
    Beighton D., Russell R.R.B.and Hayday H. (1981): The isolation and characterization of Streptococcus mutans serotype h from dental plaque of monkeys (Macaca fascicularis). J. of Gen. Microbiol., 124: 271–278.Google Scholar
  4. 4.
    Bergmeier L.A.and Lehner T. (1983): Lack of antibody to human heart tissue in sera of rhesus monkeys immunized with Streptococcus mutans antigens and comparative study with rabbit antisera. Infect. Immun., 40: 1075–1082.Google Scholar
  5. 5.
    Brandzaeg P. (1975): Immunoglobulin systems of oral mucosa and saliva. In A.E. Dolby (Ed) Oral mucosa in health and disease. Blackwell Scientific Publications, Ltd Oxford. 123–213.Google Scholar
  6. 6.
    Brahall D. (1972): Demonstration of five serological groups of streptococcal strains resembling Streptococcus mutans. Odont. Revy, 21: 143–152.Google Scholar
  7. 7.
    Bruyère T., Wachsmann D., Klein J.P., Schöller M. and Frank R.M. (1987): Local response in rat to liposome associated Streptococcus mutans polysaccharide-protein conjugate. Vaccine, 5: 39–42.Google Scholar
  8. 8.
    Challacombe S.J. and Lehner T. (1979): Salivary antibody responses in rhesus monkeys immunized with Streptococcus mutans by the oral, submucosal or subcutaneous route. Arch. oral Biol., 24: 917–925.Google Scholar
  9. 9.
    Clark W.B. and Gibbons R.J. (1977). Influence of salivary components and extracellular polysaccharide synthesis from sucrose on the attachment of Streptococcus mutans 6715 to hydroxyapatite surface. Infect. Immun., 18: 514–523.Google Scholar
  10. 10.
    Coykendall A.L. and Gustafson K.B. (1986). Taxonomy of Streptococcus mutans. In: Molecular microbiology and immunobiolgy of Streptococcus mutans. Hamada S., Michalek S.M., Kiyono H., Menaker L. and McGhee J.R. (Eds). Elsevier Science Publishers Amsterdam, New-York, Oxford.Google Scholar
  11. 11.
    Curtiss III R. (1986): Genetic analysis of Streptococcus mutans virulence and prospects for an anticaries vaccine. J. Dent. Res., 65: 1034–1045.Google Scholar
  12. 12.
    Delacroix D.L., Dive C, Rambaud J. C. and Vaerman J.P. (1982): IgA subclasses in various secretions and in serum. Immunology, 47: 383–385.Google Scholar
  13. 13.
    Douglas C.W.I. and Russell R.R.B. (1985). Effect of specific antisera upon Streptococcus mutans adherence to saliva coated hydroxyapatite. FEMS Microbiol. Lett., 25: 211–214.Google Scholar
  14. 14.
    Emmings F.G., Evans R.T. and Genco R.J. (1975): Antibody response in the parotid fluid and serum of irus monkeys (Macaca fascicularis) after local immunization with S. mutans. Infect. Immun., 12: 281–292.Google Scholar
  15. 15.
    Evans R.T., Emmings F.G. and Genco R.J. (1975): Prevention of Streptococcus mutans infection of tooth surfaces by salivary antibody in irus monkeys. Infect. Immun., 12: 293–302.Google Scholar
  16. 16.
    Everhart D.L., Mallett C.P. and Doyle G. (1985): Dental caries vaccine: some problems solved. Microbial Sciences, 10: 312–313.Google Scholar
  17. 17.
    Ferretti J.J., Shea C. and Humphrey M.W. (1980): Cross reactivity of Streptococcus mutans antigens and human heart tissue. Infect. Immun., 30: 69–73.Google Scholar
  18. 18.
    Gahnberg L. and Krasse B. (1983): Salivary immunoglobulin A antibodies and recovery from challenge of Streptococcus mutans after oral administration of Streptococcus mutans vaccine in humans. Infect. Immun., 39: 514–519.Google Scholar
  19. 19.
    Genco R.J., Linzer R. and Evans R.T. (1983): Effect of adjuvants on orally administered antigens. Ann. N.Y. Acad. Sci., 409: 650–667.Google Scholar
  20. 20.
    Gibbons R.J. and Etherden I. (1983): Comparative hydrophobicities of oral bacteria and their adherence to salivary pellicles. Infect. Immun., 41: 1190–1196.Google Scholar
  21. 21.
    Gibbons R.J. and Van Houte J. (1975): Dental caries. Ann. Rev. Med., 26: 121–136.Google Scholar
  22. 22.
    Gregory R.L., Filler S.J., Michalek S.M. and McGhee J.R. (1986): Salivary immunoglobulin A and serum antibodies to Streptococcus mutans ribosomal preparations in dental caries free and caries susceptible human subjects. Infect. Immun., 51:348–351.Google Scholar
  23. 23.
    Gregory R.L., Michalek S.M., Filler S.J., Mestecky J.and McGhee J.R. (1985): Prevention of Streptococcus mutans colonization by salivary IgA. J. Clin. Immunol., 5: 55–62.Google Scholar
  24. 24.
    Gregory R.L., Michalek S.M., Shechmeister I.L. and McGhee J.R. (1983): Effective immunity to dental caries: protection of gnotobiotic rats by local immunization with a ribosomal preparation from Streptococcus mutans. Microbiol. Immunol., 27: 787–800.Google Scholar
  25. 25.
    Gregory R.L., Michalek S.M., Shechmeister I.L. and McGhee J.R. (1984): Function of anti Streptococcus mutans antibodies: anti-ribosomal antibodies inhibit acid production, growth and glucose phosphotransferase activity. Infect. Immun., 45: 286–289.Google Scholar
  26. 26.
    Gregory R.L., Schöller M., Filler S.J., Crago S.S., Prince S.J., Allansmith M.R., Michalek S.M., Mestecky J. and McGhee J.R. (1985). IgA antibodies to oral and ocular bacteria in human external secretions. Prot. Biol. Fluids., 32: 53–56.Google Scholar
  27. 27.
    Gregory R.L. and Shechmeister (1983): Analysis of cell wall and membrane contamination of ribosomal preparations from Streptococcus mutans. Infect. Immun. 39: 807–816.Google Scholar
  28. 28.
    Hamada S. and Slade H.D. (1980): Biology, immunology and cariogenicity of Streptococcus mutans. Microbial Rev., 44: 331–384.Google Scholar
  29. 29.
    29.Heremans J.F. (1974): Immunoglobulin A. In: The antigens (vol. 2) (Ed. Sela M.) Academic Press New York. 365–522.Google Scholar
  30. 30.
    Hughes M., MacHardy S., Sheppard A. and Woods N. (1980): Evidence for an immunological relationship between Streptococcus mutans and human cardiac tissue. Infect. Immun., 27: 576–588.Google Scholar
  31. 31.
    Holt R.C., Abiko Y., Saito S., Smorawinska M., Hansen J.B. and Curtiss R. III. (1982): Streptococcus mutans genes that code for extracellular proteins in Escherichia coli. K12. Infect. Immun., 38: 147–156.Google Scholar
  32. 32.
    Knox K. W., and Wicken A.J. (1985). Environmentally induced changes in the surface of oral streptococci and lactobacilli. In: Molecular basis of oral adhesion. (Eds Mergenhagen S.E., and Rosan B.). American Society for microbiology. Washington D.C.Google Scholar
  33. 33.
    Lehner T., Caldwell J. and Smith R. (1985). Local passive immunization by monoclonal antibodies against streptococcal antigen I/II in the prevention of dental caries. Infect. Immun., 50: 796–799.Google Scholar
  34. 34.
    Lehner T., Challacombe S.J., and Caldwell J. (1975): An immunological investigation into the prevention of caries in deciduous teeth of rhesus monkeys. Archs oral Biol., 20: 305–310.Google Scholar
  35. 35.
    Lehner T., Challacombe S.J. and Caldwell J. (1975): Immunological and bacteriological basis for vaccination against dental caries in rhesus monkeys. Nature (London) 254: 517–520.Google Scholar
  36. 36.
    Lehner T., Challacombe S.J. and Caldwell J. (1980): Oral immunization with Streptococcus mutans in rhesus monkeys and the development of immune response to dental caries. Immunology, 41: 857–864.Google Scholar
  37. 37.
    Lehner T., Mehlert A. and Caldwell J. (1986): Local active gingival immunization by a 3,800 molecular weight streptococcal antigen in protection against dental caries. Infect. Immun., 52: 682–687.Google Scholar
  38. 38.
    Lehner T., Russell M. W., Caldwell J. and Smith R. (1981): Immunization with purified protein antigens from Streptococcus mutans against dental caries in rhesus monkeys. Infect. Immun., 34: 407–415.Google Scholar
  39. 39.
    Lehner T., Russell M. W., Challacombe S.J., Scully C.M. and Hawkers J. (1978): Passive immunization with serum and immunoglobulins against dental caries in rhesus monkeys. Lancet, i: 693–695.Google Scholar
  40. 40.
    McGhee J.R.,and Michalek S.M. (1981): Immunobiology of dental caries: microbial aspect and local immunity. Ann. Rev. Microbiol., 35: 595–638.Google Scholar
  41. 41.
    McGhee J.R., Michalek S.M., Week J., Navia J.M., Rahmen A.F.R. and Legler D. W. (1975): Effective immunity to dental caries: protection of gnotobiotic rats by local immunization with Streptococcus mutans J. Immunol., 114: 300–305.Google Scholar
  42. 42.
    Mestecky J., Czerkinsky C., Brown T.A., Prince S.J., Michalek S.M., Russell M. W., Jackson S., Schöller M. and McGhee J.R. (1986): Human immune responses to Streptococcus mutans. In: Molecular microbiology and immunobiology of Streptococcus mutans (Eds. Hamada S., Michalek S.M., Kiyono H., Menaker L. and McGhee J.R.). Elsevier Science Publishers. Amsterdam, New-York, Oxford.Google Scholar
  43. 43.
    Mestecky J., McGhee J.R., Arnold R.R., Michalek S.M., Prince S.J. and Babb J.L. (1978): Selective induction of an immune response in human external secretions by ingestion of bacterial antigens. J. Clin. Invest., 61: 731–737.Google Scholar
  44. 44.
    Mestecky J., McGhee J.R., Michalek S.M., Anold R.R., Crago S.S. and Babb J.L. (1978): Concept of the local and common mucosal immune response. Adv. Exp. Med. Biol., 107: 185–196.Google Scholar
  45. 45.
    Michalek S.M., McGhee J.R., Arnold R.R. and Mestecky J. (1978): Effective immunity to dental caries: selective induction of secretory immunity by oral administration of Streptococcus mutans. In Secretory immunity and infection (Eds. McGhee J.R. and Mestecky J.). Plenum Publishing corporation, city.Google Scholar
  46. 46.
    Michalek S.M., McGhee J.R., Mestecky J., Arnold R.R. and Bozzo L. (1976): Ingestion of Streptococcus mutans induces secretory immunoglobulin A and caries immunity. Science, 192: 1238–1240.Google Scholar
  47. 47.
    Michalek S.M., Morisaki I., Gregory R.L., Kimura S., Harmon C.C., Hamada S., Kotani S. and McGhee J.R. (1985): Oral adjuvants enhance salivary IgA response to purified Streptococcus mutans antigens. Prot. Biol. Fluids, 32: 47–52.Google Scholar
  48. 48.
    Michalek S.M., Morisaki I., Harmon C.C., Hamada S. and McGhee J.R. (1983): Effective immunity to dental caries: gastric intubation of Streptococcus mutans whole cells or cell walls induces protective immunity in gnotobiotic rats. Infect. Immun., 39: 645–654.Google Scholar
  49. 49.
    Morisaki I., Michalek S.M., Harmon C.C., Torii M., Hamada S. and McGhee J.R. (1983): Effective immunity to dental caries: enhancement of salivary anti Streptococcus mutans antibody response with oral adjuvants. Infect. Immun., 40: 577–591.Google Scholar
  50. 50.
    Mukasa H. and Slade H.D. (1974): Mechanism of adherence of Streptococcus mutans to smooth surface. II. Nature of the binding site and the adsorption of dextran, levan synthetase enzymes on the cell wall surface of Streptococcus. Immun., 9: 419–429.Google Scholar
  51. 51.
    Ogier J.A., Bruyere T., Ackermans F., Klein J.P. and Frank R.M. (1985): Specific inhibition of Streptococcus mutans interactions with saliva components by monoclonal antibodies binding to different epitopes on the 74K cell wall saliva receptor (74KSR). FEMS Microbiol. Lett., 30: 233–237.Google Scholar
  52. 52.
    Ogier J.A., Klein J.P., Sommer P. and Frank R.M. (1984): Identification and preliminary characterization of saliva interacting antigens of Streptococcus mutans by immunoblotting, ligand blotting and immunoprecipitation. Infect. Immun., 45: 107–112.Google Scholar
  53. 53.
    Perch B., Kjems E. and Ravn T. (1975): Biochemical properties of Streptococcus mutans from various human and animal sources. Acta. Pathol. Microbiol. Scand., 82: 357–370.Google Scholar
  54. 54.
    Pucci M.J., Tew J. G. and Macrina F.L. (1986): Human serum antibody response against Streptococcus mutans antigens. Infect. Immun., 51: 600–606.Google Scholar
  55. 55.
    Robeson J.P., Barletta R. G. and Curtiss III R. (1983): Expression of a Streptococcus mutans glucosyltrasferase gene in Escherichia coli. J. Bact., 152: 211–221.Google Scholar
  56. 56.
    Russell M. W. (1986): Protein antigens of Streptococcus mutans. In: Molecular microbiology and immunobiology of Streptococcus mutans (Eds. Hamada S., Michalek S.M., Kiyono H., Menaker L. and McGhee J.R.). Elsevier Science Publishers. Amsterdam, New-York, Oxford.Google Scholar
  57. 57.
    Russell M. W. and Mestecky J. (1986): Potential for immunological intervention against dental caries. J. Biol. Buccale., 14: 159–175.Google Scholar
  58. 58.
    Russell R.R.B. (1979): Glucan binding proteins of Streptococcus mutans serotype c. J. Gen. Microbiol., 112: 117–201.Google Scholar
  59. 59.
    Russell R.R.B., Beighton D. and Cohen B. (1982): Immunization of monkeys Macaca fascicularis with antigen purified from Streptococcus mutans. Brit. Dent. J., 152: 81–84.Google Scholar
  60. 60.
    Smith D.J., Gahnberg L., Taubman M.A. and Ebersole J.L. (1986): Antibody to streptococcal antigens in young adults and children. In: Molecular microbiology and immunobiology of Streptococcus mutans (Eds. Hamada S., Michalek S., Kiyono H., Menaker L. and McGhee J.R.). Elsevier Science Publishers. Amsterdam, New-York, Oxford.Google Scholar
  61. 61.
    Smith D.J., Taubman M.A., and Ebersole J.L. (1979): Effect of oral administration of glucosyltransferases antigen on experimental dental caries. Infect. Immun., 26: 82–89.Google Scholar
  62. 62.
    Smith D.J., Taubman M.A., and Ebersole J.L. (1980): Local and systemic antibody response to oral administration of glucosyltransferase antigen complex. Infect. Immun., 28: 441–450.Google Scholar
  63. 63.
    Stinson M. W., Barna P.K., Bergey E.J., Nisengard R.J., Neiders M.E. and Albini B. (1984): Binding of Streptococcus mutans antigens to heart and kidney basement membranes. Infect. Immun., 46: 145–151.Google Scholar
  64. 64.
    Stiles H.M.,Meyers R., Brunelle J.A. and Wittig A.B. (1976) Occurrence of Streptococcus mutans and Streptococcus saguis in the oral cavity and feces of young children. In: Proceedings: microbial aspect of dental caries (special supplement to microbiology abstracts, vol. 1), (Eds. Stiles H.M., Loesche W.J., O'Brien T.C.). Information retrieval, Washington D.C: 187–199.Google Scholar
  65. 65.
    Taubman M.A., and Smith D.J. (1974): Effect of local immunization with Streptococcus mutans on induction of salivary immunoglobulin A,antibody and experimental caries in rats. Infect. Immun., 9: 1079–1091.Google Scholar
  66. 66.
    Taubman M.A. and Smith D.J. (1977): Effect of local immunization with glucosyltransferases fractions from Streptococcus mutans on dental caries in rats and hamsters. J. Immunol., 118: 710–720.Google Scholar
  67. 67.
    Van de Rijn L, Bleiweis A.S. and Zabriskie J.B. (1976): Antigens in Streptococcus mutans cross reactive with human heart muscle. J. Dent. Res., 55: 59–64.Google Scholar
  68. 68.
    Wachsmann D., Klein J.P., Schöller M. and Frank R.M. (1985): Local and systemic immune response to orally administered liposome associated soluble S. mutans cell wall antigens. Immunology, 54: 189–194.Google Scholar
  69. 69.
    Wachsmann D., Klein J.P., Schöller M., Ogier J.A., Ackermans F. and Frank R.M (1986): Serum and salivary antibody responses in rats orally immunized with Streptococcus mutans carbohydrate protein conjugate associated with liposomes. Infect. Immun., 52: 408–413.Google Scholar
  70. 70.
    Walker J. (1981): Antibody responses of monkeys oral and local immunization with Streptococcus mutans. Infect. Immun., 31: 61–70.Google Scholar

Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • P. Klein
    • 1
  • M. Scholler
    • 1
  1. 1.Unitè de Recherches U 157, Institut National de la Santé et de la Recherche MédicaleFaculté de Chirurgie DentaireStrasbourgFrance

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