Biotechnology Letters

, Volume 4, Issue 3, pp 159–164

Production of l-lactic acid with immobilized Lactobacillus delbrueckii

  • Sirkka-Liisa Stenroos
  • Yu-Yen Linko
  • P. Linko
Article

Summary

Living Lactobacillus delbrueckii cells were entrapped in calcium alginate gel beads and employed both in recycle batch and continuous column reactors to produce l-lactic acid from glucose. The substrate contained l% (w/v) yeast extract as nutrient and 4.8% (w/v) solid calcium carbonate as buffer. The maxiumum lactic acid yield obtained was 97%, of which more than 90% was l-lactic acid. The biocatalyst activity half-life in continuous operation was about 100 d, and only about 10% of the activity was lost during intermittent storage of the bioreactor at +7°C for about 5 months.

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Copyright information

© Science and Technology Letters 1981

Authors and Affiliations

  • Sirkka-Liisa Stenroos
    • 1
  • Yu-Yen Linko
    • 1
  • P. Linko
    • 1
  1. 1.Department of ChemistryHelsinki University of TechnologyEspooFinland

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