Clinical & Experimental Metastasis

, Volume 13, Issue 1, pp 49–56

Overexpression and localization of cathepsin B during the progression of human gliomas

  • Marupudi Sivaparvathi
  • Raymond Sawaya
  • Shang Wu Wang
  • Alan Rayford
  • Masaaki Yamamoto
  • Lance A. Liottat
  • Garth L. Nicolson
  • Jasti S. Rao
Research Papers

Abstract

Degradation of the extracellular matrix is a prerequisite for acquisition of the invasive phenotype. Several proteinases released by invading tumor cells appear to participate in the focal degradation of extracellular matrix proteins. Using an enzyme-linked immunosorbent assay, enzymatic assays, Western and Nothern blotting techniques, we determined whether increased levels of the cysteine protease cathepsin B correlated with the progression and invasion of human gliomas. The amount of cathepsin B activity and protein content were highest in glioblastomas, lower in anaplastic astrocytomas and lowest in normal brain tissue and low-grade gliomas. There were significantly higher amounts of Mr 25 000 and 26 000 bands in glioblastoma and anaplastic astrocytoma than in normal brain and low-grade glioma tissue extracts as determined by Western blotting with anti-cathepsin antibodies. In addition, cathepsin B transcripts were overexpressed in anaplastic astrocytoma (about two- to three-fold), in glioblastoma (about eight- to 10-fold), compared with normal brain tissue and low-grade glioma. Immunobistochemical staining for cathepsin B showed intense immunoreactivity in tumor and endothelial cells of glioblastomas and anaplastic astrocytomas but only weak immunoreactivity in low-grade glioma and normal brain tissues. Therefore, we conclude that cathepsin B expression is greatest in highly malignant astrocytomas, especially in glioblastomas, and is correlated with the malignant progression of astrocytomas.

Keywords

cysteine proteases extracellular matrix glioblastoma multiforme invasion 

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Copyright information

© Rapid Communications of Oxford Ltd 1995

Authors and Affiliations

  • Marupudi Sivaparvathi
    • 1
  • Raymond Sawaya
    • 1
  • Shang Wu Wang
    • 1
  • Alan Rayford
    • 1
  • Masaaki Yamamoto
    • 1
  • Lance A. Liottat
    • 3
  • Garth L. Nicolson
    • 2
  • Jasti S. Rao
    • 1
  1. 1.Department of NeurosurgeryThe University of Texas M. D. Anderson Cancer CenterHoustonUSA
  2. 2.Department of Tumor BiologyThe University of Texas M. D. Anderson Cancer CenterHoustonUSA
  3. 3.Laboratory of PathologyNCIBethesdaUSA
  4. 4.Department of NeurosurgeryThe University of Texas M. D. Anderson Cancer CenterHoustonUSA

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