Biotechnology Letters

, Volume 18, Issue 3, pp 269–274

Cloning and expression of the Clostridium thermosulfurogenes D-xylose isomerase gene (xyLA) in Saccharomyces cerevisiae

  • Catharine J. Moes
  • Isak S. Pretorius
  • Willem H. van Zyl
Article

Summary

A recombinant strain of Saccharomyces cerevisiae containing the bacterial d-xylose isomerase (xylA) gene of Clostridium thermosulfurogenes was constructed. The xylA gene has high thermal stability, functions in an anaerobic environment and has a G+C content of 38.9%, which should render it a good candidate for successful expression in S. cerevisiae. Efficient transcription of the xylA gene under the control of the ADH2 gene promoter and terminator sequences in a recombinant S. cerevisiae strain was shown through Northern blot analysis. However, the xylA recombinant strain was unable to grow on d-xylose as sole carbon source.

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Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • Catharine J. Moes
    • 1
  • Isak S. Pretorius
    • 1
  • Willem H. van Zyl
    • 1
  1. 1.Department of MicrobiologyUniversity of StellenboschStellenboschRepublic of South Africa

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