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Biotechnology Letters

, Volume 18, Issue 3, pp 269–274 | Cite as

Cloning and expression of the Clostridium thermosulfurogenes D-xylose isomerase gene (xyLA) in Saccharomyces cerevisiae

  • Catharine J. Moes
  • Isak S. Pretorius
  • Willem H. van Zyl
Article

Summary

A recombinant strain of Saccharomyces cerevisiae containing the bacterial d-xylose isomerase (xylA) gene of Clostridium thermosulfurogenes was constructed. The xylA gene has high thermal stability, functions in an anaerobic environment and has a G+C content of 38.9%, which should render it a good candidate for successful expression in S. cerevisiae. Efficient transcription of the xylA gene under the control of the ADH2 gene promoter and terminator sequences in a recombinant S. cerevisiae strain was shown through Northern blot analysis. However, the xylA recombinant strain was unable to grow on d-xylose as sole carbon source.

Keywords

Thermal Stability Saccharomyces Cerevisiae Northern Blot Gene Promoter Clostridium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • Catharine J. Moes
    • 1
  • Isak S. Pretorius
    • 1
  • Willem H. van Zyl
    • 1
  1. 1.Department of MicrobiologyUniversity of StellenboschStellenboschRepublic of South Africa

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