, Volume 7, Issue 2, pp 201–208

Silver, copper, lead and zinc accumulation byPseudomonas stutzeri AG259 andStreptomyces albus : electron microscopy and energy dispersive X-ray studies

  • B. Mattuschka
  • G. Straube
  • J. T. Trevors
Research Papers


Metal accumulation by a silver-resistant Pseudomonas stutzeri AG259 strain and a Streptomyces albus strain was investigated in a mixed metal solution of silver, copper, lead and zinc. The location of silver, lead and copper on cells was determined by transmission electron microscopy coupled with an X-ray analysis system. In P. stutzeri cells silver was detected as dense deposits on the cells. Copper and lead were distributed over the cells. S. albus accumulated these metals only on part of cells with a higher concentration per cell than in P. stutzeri.


accumulation copper electron microscopy lead Pseudomonas stutzeri silver Streptomyces albus X-ray analysis zinc 


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  1. Aiking H, Stijnman A, van Garderen C, van Heerikuizen H, van't Riet. 1984 Inorganic phosphate accumulation and cadmium detoxification in Klebsiella aerogenes NCTC 418 growing in continuous culture. Appl Environ Microbiol 47, 374–377.Google Scholar
  2. Beveridge TJ. 1986 The immobilization of soluble metals by bacterial walls. Biotechnol Bioeng Symp 16, 127–139.Google Scholar
  3. Beveridge TJ, Murray KGE. (1976) Uptake and retention of metals by cell walls of Bacillus subtilis. J Bacteriol 127, 1502–1518.Google Scholar
  4. Chandler JA. 1977 Wavelength dispersive X-ray microanalysis in biological research. In: Meek GA, Elder HY, eds Analytical and Quantitative Methods in Microscopy. Cambridge: Cambridge University Press; 227–240.Google Scholar
  5. Cotoras D, Viedma P, Cifuentes L, Mestre A. 1992 Sorption of metal ions by whole cells of Bacillus and Micrococcus. Environ Technol 13, 551–559.Google Scholar
  6. Farah KS, Sneddon J. 1992 Selective removal of silver in the presence of copper, manganese, and zinc in solution by an algal biomass. J Environ Sci Health A27, 1579–1587.Google Scholar
  7. Ford T, Mitchell R. 1992 Microbial transport of toxic metals. In: Mitchell R, ed. Environmental Microbiology. Wiley-Liss Inc, 83–101.Google Scholar
  8. Friis N, Myers-Keith P. 1986 Biosorption of uranium and lead by Streptomyces longwoodensis. Biotechnol Bioeng 28, 21–28.Google Scholar
  9. Gadd GM. (1989) Accumulation of metals by microorganisms and algae. In: Rehm HJ, ed. Microbial Processes. Weinheim: VCG Verlag.Google Scholar
  10. Goddard PA, Bull AT. 1989 Accumulation of silver by growing and non-growing populations of Citrobacter intermedius B6. Appl Microbiol Biotechnol 31, 314–319.Google Scholar
  11. Haefeli C, Franklin C, Hardy K. 1984 Plasmid-determined silver resistance in Pseudomonas stutzeri isolated from a silver mine. J Bacteriol 158, 389–392.Google Scholar
  12. Hughes MN, Poole RK. 1989 Metal mimicry and metal limitation in studies of metal-microbe interactions. In: Poole RK, Gadd GM, eds. Metal-microbe Interactions. Oxford: IRL Press, 1–17.Google Scholar
  13. Hutchins SR, Davidson MS, Brierley JA, Brierley CL. 1986 Microorganisms in reclamation of metals. Annu Rev Microbiol 40, 311–336.Google Scholar
  14. Junghans K, Straube G. 1991 Uptake of copper by yeasts. Biol Met 4, 233–237.Google Scholar
  15. Klapcinska B, Chmielowski J. 1986 Binding of germanium to Pseudomonas putida cells. Appl Envion Microbiol 51, 1144–1147.Google Scholar
  16. Kuyucak N, Volesky B. 1988 Biosorbents for recovery of metals from industrial solutions. Biotechnol Lett 10, 137–142.Google Scholar
  17. Macaskie LE, Dean ACR, Cheetham AK, Jakeman RJB, Skarnulis AJ. 1987 Cadmium accumulation by Citrobacter sp.: the nature of the accumulated metal precipitate and its location on the bacterial cells. J Gen Microbiol 133, 539–544.Google Scholar
  18. Mullen MD, Wolf DC, Ferris FG, et al. 1989 Bacteriol Sorption of heavy metals. Appl Environ Microbiol 55, 3143–3149.Google Scholar
  19. Nakajima A, Sakaguchi F. 1986 Selective accumulation of heavy metals by microorganisms. Appl Microbiol Biotechnol 24, 59–64.Google Scholar
  20. Nakajima A, Horikoshi T, Sakaguchi T. 1981 Studies on the accumulation of heavy metal elements in biological systems. European J Appl Microbiol Biotechnol 12, 90–96.Google Scholar
  21. Pighi L, Schinner F. 1989 Selective accumulation of silver by fungi. Biotechnol Lett 11, 275–280.Google Scholar
  22. Scott JA, Palmer SJ. 1988 Cadmium biosorption by bacterial exopolysaccharide. Biotechnol Lett 10, 21–24.Google Scholar
  23. Slawson RM, Lee H, Trevors JT. 1990 Bacteriol interactions with silver. Biol Met 3, 151–154.Google Scholar
  24. Slawson RM, Van Dyke MI, Lee H, Trevors JT. 1992a Germanium and silver resistance, accumulation and toxicity in microorganisms. Plasmid 27, 72–79.Google Scholar
  25. Slawson RM, Trevors JT, Lee H. 1992b Silver accumulation and resistance in Pseudomonas stutzeri. Arch Microbiol 158, 398–404.Google Scholar
  26. Starodub ME, Trevors JT. 1989 Silver resistance in Escherichia coli R1. J Med Microbiol 29, 101–110.Google Scholar
  27. Trevors JT, Cotter CM. 1990 Copper toxicity and uptake in microorganisms. J Ind Microbiol 6, 77–84.Google Scholar
  28. White C, Gadd GM. 1990 Biosorption of radionuclides by fungal biomass. J Chem Tech Biotechnol 49, 331–343.Google Scholar

Copyright information

© Rapid Communications of Oxford Ltd 1994

Authors and Affiliations

  • B. Mattuschka
    • 1
    • 3
  • G. Straube
    • 1
  • J. T. Trevors
    • 1
    • 2
  1. 1.Institute of Bioengineering, Martin-Luther University of HalleMerseburgGermany
  2. 2.Department of Environmental BiologyUniversity of GuelphOntarioCanada
  3. 3.Institute of Bioengineering, Martin-Luther University of HalleMerseburgGermany

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