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Biotechnology Letters

, Volume 18, Issue 5, pp 537–540 | Cite as

Production of cellulase and β-glucosidase activity during growth of the actinomycete Micromonospora chalcae on cellulose-containing media

  • J. Gallagher
  • A. Winters
  • N. Barron
  • L. McHale
  • A. P. McHale
Article

Abstract

The actinomycete, Micromonospora chalcae produced an extracellular cellulase system when grown on cellulose-containing media. Cellulase activity reached a maximum after 8 days and the β-glucosidase activity was maximal within 16 to 18 days. β-Glucosidase activity was isolated and had a Km of 0.25mM for the substrate p-nitrophenyl-β-D-glucoside. The activity was optimal at pH 7.25 and 52°C.

Keywords

Cellulase Cellulase Activity Cellulase Production Dyfed Glucosidase Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • J. Gallagher
    • 2
    • 1
  • A. Winters
    • 2
    • 1
  • N. Barron
    • 1
  • L. McHale
    • 1
  • A. P. McHale
    • 1
  1. 1.Biotechnology Research Group, School of Applied Biological Sciences, University of UlsterColeraineNorthern Ireland
  2. 2.Inst. of Grasslands and Environmental ResearchAberystwythWales

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