Biotechnology Letters

, Volume 18, Issue 1, pp 63–68 | Cite as

Effect of culture conditions on the aldehyde dehydrogenase activity of Acetobacter aceti cytoplasmatic extracts

  • A. Blandino
  • I. Caro
  • D. Cantero
Article

Summary

The aldehyde dehydrogenase activity of the cytoplasmatic extracts of a Acetobacter aceti strain used industrially in vinegar production is investigated. Two different culture media, based on ethanol or glucose, were used to compare the strain growth and activity. The kinetic constants of the enzymatic activity have been determined for extracts from the strain grown on ethanol. Finally, a study has been made of the spontaneous enzyme de-activation, which seems to fit a first order kinetic.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Adachi, O., Miyagawa, E., Shinagawa, E., Matsushita, K. and Ameyama, M. (1978). Agric. Biol. Chem. 42, 2331–2340.Google Scholar
  2. Adachi, O., Tayama, K., Shinagawa, E., Matsushita, K. and Ameyama, M. (1980). Agric. Biol. Chem. 42, 503–515.Google Scholar
  3. Adachi, O., Tayama, K., Shinagawa, E., Matsushita K. and Ameyama, M. (1987). Agric. Biol. Chem. 42, 2045–205.Google Scholar
  4. Ameyama, M., Osada, K., Shinagawa, E., Matsushita, K. and Adachi, O. (1981). Agric. Biol. Chem. 45, 1889–1890.Google Scholar
  5. Dixon, M. and Webb, E.C. (1979) In: Enzymes, Longman Group Ltd, 3rd. ed. New York.Google Scholar
  6. Lencki, R.W., Arul, J. and Neufeld, R.J. (1992). Biotechnol. Bioeng. 40, 1421–1426.Google Scholar
  7. Muraoka, H., Watabe, Y., Ogasawara, N. and Takahashi, H. (1980). J. Ferment. Technol. 58(6), 501–507.Google Scholar
  8. Muraoka, H., Watabe, Y., Ogasawara, N. and Takahashi, H. (1981a).J. Ferment. Technol. 59(4), 247–255.Google Scholar
  9. Muraoka, H., Watabe, Y., Ogasawara, N. and Takahashi, H. (1981b). J. Ferment. Technol. 60(1), 41–50.Google Scholar
  10. Muraoka, H., Watabe, Y. and Ogasawara, N. (1982). J. Ferment. Technol. 62(2), 125–130.Google Scholar
  11. Nanba, A., Tamura, A. and Nagai, S. (1984). J. Ferment. Technol. 62(6), 501–505.Google Scholar
  12. Romero, L.E., Gómez, J.M., Caro, I. and Cantero, D. (1994). Chem. Engi. J. 54, B15-B24.Google Scholar
  13. Smith, J.M., (1972). In: Chemical Engineerig Kinetics, Mc Graw-Hill Book Company 2rd. ed. New York.Google Scholar
  14. Swings, J. and De ley, J., (1984). In: Bergey's Manual of systematic Bacteriology (9th ed.). I. de Holt, J.G. Ed. The Williams and Wilkins Co., Baltimore.Google Scholar

Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • A. Blandino
    • 1
  • I. Caro
    • 1
  • D. Cantero
    • 1
  1. 1.Biological and Enzimatic Reactors Group Department of Chemical Engineering, Faculty of SciencesUniversity of Cádiz (UCA)Puerto RealSpain

Personalised recommendations