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Biotechnology Letters

, Volume 2, Issue 1, pp 29–34 | Cite as

Immobilized bacterial cells containing a thermostable β-galactosidase

  • M. De Rosa
  • A. Gambacorta
  • B. Nicolaus
  • V. Buonocore
  • E. Poerio
Article

Summary

A constitutive β-galactosidase has been localized in the cytosol of thermoacidophilic bacterium Caldariella acidophila. Cells have been entrapped in polyacrylamide gel with full retention of enzymic activity; no activity decrease is observed after 8 months of storage. Enzyme properties in entrapped cells are similar to those of the free enzyme. A 73% hydrolysis of lactose has been achieved in a continuous system on a 2 ml entrapped cell column operating at 70°C; half life in these conditions is 30 days.

Keywords

Enzyme Hydrolysis Enzymic Activity Organic Chemistry Half Life 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Science and Technology Letters 1979

Authors and Affiliations

  • M. De Rosa
    • 1
  • A. Gambacorta
    • 1
  • B. Nicolaus
    • 1
  • V. Buonocore
    • 2
  • E. Poerio
    • 2
  1. 1.Laboratorio per la Chimica di Molecole di Interesse Biologico del C.N.R.NapoliItaly
  2. 2.Istituto di Chimica Organica e Biologica, Università di NapoliItaly

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