Biotechnology Letters

, Volume 16, Issue 7, pp 737–740

Production of ethanol by the thermotolerant yeastKluyveromyces marxianus IMB3 during growth on lactose-containing media

  • D. Brady
  • R. Marchant
  • L. McHale
  • A. P. McHale
Article

Summary

The thermotolerant yeast strain,Kluyveromyces marxianus IMB3 was shown to be capable of growth and ethanol production on lactose containing media at 45°C. On media containing 4% (w/v) lactose, ethanol production increased to 6.0g/l within 50h and this represented 29% of theoretical yield. During growth on lactose containing media the organism was shown to produce a cell-associated β-galactosidase and no significant enzyme could be detected in the extracellular culture filtrate. Addition of β-galactosidase, released fromKluyveromyces marxianus IMB3 cells, to active fermentations, resulted in increasing ethanol production to 53% of theoretical yield at 45°C.

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Copyright information

© Chapman & Hall 1994

Authors and Affiliations

  • D. Brady
    • 1
  • R. Marchant
    • 1
  • L. McHale
    • 1
  • A. P. McHale
    • 1
  1. 1.Biotechnology Research Group, School of Applied Biological and Chemical Sciences, University of UlsterColeraineNorthern Ireland

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