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Biotechnology Letters

, Volume 16, Issue 7, pp 697–702 | Cite as

A comparison of different strategies for lipase-catalyzed synthesis of partial glycerides

  • U. Bornscheuer
  • H. Stamatis
  • A. Xenakis
  • T. Yamane
  • F. N. Kolisis
Article

Summary

Four different approaches for the synthesis of monolaurylglycerol (MLG) by non specificPseudomonas cepacia lipase in a crude and purified form have been studied: a. The direct esterification of glycerol by lauric acid in bis-(2-ethylhexyl)sulfosuccinate sodium salt (AOT)/isooctane microemulsion systems; b. the transesterification of glycerol by vinyl laurate in the presence or not of any solvent; c. solid-phase glycerolysis of trilaurin; and, d. transesterification of protected glycerol, 1,2-O-isopropylidene glycerol, by vinyl laurate, in the presence or not of any solvent. It was found that in the two latter cases (d and c) the formation of pure MLG was occurred, while in the first two cases (a and b) apart of MLG the formation of DLG was also observed.

Keywords

Lipase Lauric Acid Microemulsion System Trilaurin Crude Lipase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Akoh, C.C. 1993.Biotech. Lett.,15, 949–954.CrossRefGoogle Scholar
  2. Bornscheuer, U. T., Yamane, T.Enz. Microbiol. Technol., in press.Google Scholar
  3. Chang, P.S., Rhee, J.S., Kim, J.J. 1991.Biotechnol. Bioeng.,38, 1159–65.CrossRefGoogle Scholar
  4. Hartmann, L. 1959.J. Chem. Soc.,, 4134.Google Scholar
  5. Hayes, D.G., Gulari, E. 1992.Biotechnol. Bioeng.,40, 110–118.CrossRefGoogle Scholar
  6. Hayes, D.G., Gulari, E. 1991.Biotechnol. Bioeng.,38, 507–517.CrossRefGoogle Scholar
  7. Hess, R., Bornscheuer, U., Capewell, A., Scheper, T.,Enz. Microbiol. Technol., submittedGoogle Scholar
  8. Holmberg, K., Larssen, B., Stark, M.B. 1988.J. Am. Oil Chem. Soc.,66, 1796–1800.Google Scholar
  9. McNeill, G.P., Shimizu, S., Yamane, T. 1990.J. Am. Oil Chem. Soc.,67, 779–783.Google Scholar
  10. Stamatis, H., Xenakis, A. Provelegiou, M., Kolisis, F.N. 1993.Biotechnol. Bioeng.,42, 103–110.CrossRefGoogle Scholar
  11. Yamaguchi, S., Mase, T. 1991.J. Ferment. Bioeng.,72, 162–167.CrossRefGoogle Scholar
  12. Yamane, T., Hog, M.M., Itoh, S., Shimizu, S. 1986.J. Japan. Oil Chem. Soc,35(8), 625–631.Google Scholar

Copyright information

© Chapman & Hall 1994

Authors and Affiliations

  • U. Bornscheuer
    • 1
  • H. Stamatis
    • 2
  • A. Xenakis
    • 2
  • T. Yamane
    • 1
  • F. N. Kolisis
    • 3
  1. 1.Lab. of Molecular Biotechnology, Dept of Applied Biological ScienceSchool of Agricultural Sciences, Nagoya UniversityNagoyaJapan
  2. 2.Institute of Biological Research & Biotechnology, National Hellenic Research FoundationAthensGreece
  3. 3.Biosystems Technology Laboratory, Chemical Engineering Dept., Division IVNational Technical University of Athens, Zographou campusAthensGreece

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