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Biotechnology Letters

, Volume 16, Issue 7, pp 667–670 | Cite as

Expression of hirudin in fed-batch cultures of recombinantSaccharomyces cerevisiae

  • Dong-Hoon Lee
  • Jin-Byung Park
  • Jin-Ho Seo
  • Eui-Sung Choi
  • Sang-Ki Lee
Article

Summary

Fed-batch fermentations were performed to produce hirudin, an anticoagulant protein, from recombinantS. cerevisiae. The structural gene coding for hirudin was combined with theGAL10 promoter for controlled expression and theMFα1 signal sequence for secretion to the growth medium. Control of galactose concentration in a fed-batch mode of operation yielded 110 g/L of final cell density and 260 mg/L of maximum hirudin concentration in the medium, which corresponds to a 3.5-fold increase in cell density and a 4.1-fold enhancement in hirudin concentration compared with the batch fermentation.

Keywords

Fermentation Galactose Batch Fermentation Recombinant Yeast Final Cell Density 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Markwardt, F. 1970. Hirudin as an Inhibitor of Thrombin. p.924–932. in G.E. Perlmann and L Lorand (eds.), Methods in Enzymol., vol. 19. Academic Press, New York, London.Google Scholar
  2. 2.
    Markwardt, F., Nowak, J., Sturzebecher, J., Walsmann, P., and Vogel, G. (1984) Thromb. Haemostasis. 52: 160–170.Google Scholar
  3. 3.
    Modak, J.M., and Lim, H.C. 1989. Biotchnol. Bioeng. 33: 11–15.CrossRefGoogle Scholar
  4. 4.
    Patkar, A.Y., and Seo, J.H. 1992. Biotechnol. Bioeng. 40: 103–109.CrossRefGoogle Scholar
  5. 5.
    Patkar, A.Y., Shin, P.K., and Seo, J.H. 1990. Kor. J. Appl. Microbiol. Biotech. 18: 394–400.Google Scholar
  6. 6.
    Sohn, J.H., Lee, S.K., Choi, E.S., and Rhee, S.K. 1991. J. Microbiol. Biotechnol. 1: 266–273.Google Scholar

Copyright information

© Chapman & Hall 1994

Authors and Affiliations

  • Dong-Hoon Lee
    • 2
  • Jin-Byung Park
    • 2
  • Jin-Ho Seo
    • 2
  • Eui-Sung Choi
    • 1
    • 2
  • Sang-Ki Lee
    • 1
    • 2
  1. 1.Genetic Engineering Research Institute KISTTaejonKorea
  2. 2.Department of Food Science and TechnologySeoul National UniversitySuwonKorea

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