Biotechnology Letters

, Volume 16, Issue 3, pp 251–256

Characteristics of sisomicin fermentation supplemented with MgSO4 in stirred and air lift fermentors

  • Chul Soo Shin
  • Seong Yong Kim
  • Jae Yeong Ju


Sisomicin fermentations were carried out in stirred and air lift fermentors, and various concentrations of MgSO4 were supplemented to improve antibiotic yield. The highest antibiotic yield was obtained at 80 mM in an air lift fermentor due to effective liberation of intracellular sisomicin by MgSO4. The presence of high concentrations of MgSO4, along with shear stress due to strong agitation in the stirred fermentor, resulted in low values of cell activity, rheological parameter(n) and cell viability in the stirred fermentor, compared with the air lift fermentor. The better physiological states of cells grown in the air lift fermentor resulted in improved antibiotic yields.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Shin,C.S., Ahn B.W., Lee,S.H., Kim,S.U., and Bok,S.H.(1988). Appl.Microbiol. Biotechnol., 28,37–38.Google Scholar
  2. 2.
    Shin,C.S.,Han,S.H.,and Lee,S.H.(1989). Kor.J.Appl.Microbiol.Bioeng., 17(3), 247–251.Google Scholar
  3. 3.
    Han,S.H.(1991). Analysis of the Effects of Magnesium Sulfate on the Improvement of Sisomicin Yield. M.S.Thesis, Yonsei University, Seoul, Korea.Google Scholar
  4. 4.
    Kim,S.Y.(1992). Characteristics of Sisomicin Fermentation Using Air Lift Fermentor. M.S.Thesis, Yonsei University, Seoul, Korea.Google Scholar
  5. 5.
    Weinstein,M.J.,Luedemann,G.M.,and Wagman,G.H.(1975). US Pat. 3,907,771.Google Scholar
  6. 6.
    Weinstein,M.J.,Luedemann,G.M.,Ridge,G.,and Wagman,G.H.(1974). US Pat. 3,832,286.Google Scholar
  7. 7.
    Han,S.H.,and Shin,C.S.(1992). Kor.J.Appl.Microbiol.Biotechnol., 20(2),213–218.Google Scholar
  8. 8.
    Hewitt,W.(1977). Microbiological Assay. Academic Press. New York.Google Scholar
  9. 9.
    Vairo,M.I.R.(1962). Biotechnol.Bioeng., 4,247–254.Google Scholar
  10. 10.
    Metz.B.,Kossen,N.W.F.,and vanSuijdam,J.C,(1979). Adv.Biochem.Eng., 11,103–156.Google Scholar
  11. 11.
    van Suijain,J.C.,and Metz,B.(1981). Biotechnol.Bioeng., 23,111–148.Google Scholar

Copyright information

© Lonsdale Press Ltd 1994

Authors and Affiliations

  • Chul Soo Shin
    • 1
  • Seong Yong Kim
    • 1
  • Jae Yeong Ju
    • 1
  1. 1.Department of Food and BiotechnologySchool of Engineering Yonsei UniversitySeodaemunku, SeoulKorea

Personalised recommendations