Biotechnology Letters

, Volume 5, Issue 7, pp 469–474 | Cite as

The inhibition of β-D-glucosidase (Aspergillus niger) by a factor in commercial yeast extract

  • Jonathan Woodward
  • James M. Lee
  • Glenna S. Zachry
Article

Summary

An inhibitor of β-D-glucosidase (E.C. 3.2.1.21) was found to be present in commercial yeast extract. The inhibitor was shown to be heat-labile, of low molecular weight, and unstable at acid pH. Inhibition was noncompetitive and apparently was not caused by proteinaceous material. Fractionation of the yeast extract was carried out on DEAE-Sepharose, to which the inhibitor did not bind.

Keywords

Molecular Weight Organic Chemistry Fractionation Yeast Extract Aspergillus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Chambers, J. P., Elbein, A. D., and Williams, J. C. (1982). Biochem. Biophys. Res. Commun. 107, 1490–1496.Google Scholar
  2. 2.
    Conchie, J., Hay, A. J., Strachan, I., and Levvy, G. A. (1967). Biochem. J. 102, 929–941.Google Scholar
  3. 3.
    Jones, T. H. D., and Gupta, M. (1981). Biochem. Biophys. Res. Commun. 102, 1310–1316.Google Scholar
  4. 4.
    Lee, J. M., and Woodward, J. Biotechnol. Bioeng. (in press).Google Scholar
  5. 5.
    Levvy, G. A., Hay, A. J., and Conchie, J. (1964) Biochem. J. 91, 378–384.Google Scholar
  6. 6.
    Mandels, M., and Reese, E. T. (1965). Ann. Rev. Phytopathol. 3, 85–102.Google Scholar
  7. 7.
    Nolan, R. A., and Nolan, W. G. (1972). Appl. Microbiol. 24, 290–291.Google Scholar
  8. 8.
    Reese, E. T., and Parrish, F. W. (1971). Carbohydr. Res. 18, 381–388.Google Scholar
  9. 9.
    Sinha, S. N., Ghosh, B. L., and Ghose, S. N. (1981). Can. J. Microbiol. 27, 1334–1340.Google Scholar
  10. 10.
    Woodward, J., and Wiseman, A. (1982). Enzyme Microb. Technol. 4, 73–79.Google Scholar
  11. 11.
    Woodward, J., and Wohlpart, D. L. (1982). J. Chem. Tech. Biotech. 32, 547–552.Google Scholar

Copyright information

© Science and Technology Letters 1983

Authors and Affiliations

  • Jonathan Woodward
    • 1
  • James M. Lee
    • 1
  • Glenna S. Zachry
    • 1
  1. 1.Bioprocess Research GroupChemical Technology Division Oak Ridge National LaboratoryOak Ridge

Personalised recommendations