Biotechnology Letters

, Volume 5, Issue 3, pp 169–174 | Cite as

Optimization of process design for continuous ethanol production by Zymomonas mobilis ATCC 29191

  • Robert C. Charley
  • Jared E. Fein
  • Barbara H. Lavers
  • Hugh G. Lawford
  • G. Ross Lawford
Article

Abstract

A two-stage continuous-stirred-tank-reactor (CSTR) system is used to achieve high final alcohol concentrations (>100 g/l) with Zymononas mobilis ATCC 29191. By employing continuous cell recycle on the second stage CSTR of a two-stage process a high overall volumetric productivity (18.13 g/l/h) is achieved together with >100 g/l effluent alcohol.

Nomenclature

VI

First stage operating volume (1)

VII

Second stage operating volume (1)

SR

Medium glucose concentration (g/l)

So

Effluent glucose concentration (g/l)

X

Biomass concentration (g/l)

P

Ethanol concentration (g/l)

VP

Volumetric productivity (g ethanol/l/h)

Yp/s

Product yield coefficient (g ethanol/g glucose consumed)

Qp

Specific rate of ethanol formation (g ethanol/g cells/h)

D

Dilution rate (h1)

Dmax

Maximum dilution rate: ie., highest dilution rate at which the effluent glucose concentration 4g/l (h-1)

MRT2

Mean cell residence time in second stage CSTR (h)

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References

  1. Fein, J.E., Lawford, H.G., Lawford, G.R., Zawadzki, B.C. and Charley, R.C. (1983A). Biotechnol. Letters (Accepted for publication).Google Scholar
  2. Fein, J.E., Charley, R.C., Hopkins, K.A., Lavers, B., and Lawford, H.G., (1983B). Biotechnol. Letters (Accepted for publication).Google Scholar
  3. Lawford, H.G., Lawford, G.R., Lavers, B., and Fein, J., (1982). Presented at the XIII International Congress Microbiol., (Boston, Ma.).Google Scholar
  4. Light, P.A., and Garland, P.B., (1971). Biochem. J., 124, 123–124.Google Scholar
  5. Rogers, P.L., Lee, K.J., Skotnicki, M.L., and Tribe, D.E., (1982). In: “Advances in Biochemical Engineering”, Vol. 23, Ed. A. Feichter, Springer-Verlag (New York).Google Scholar

Copyright information

© Science and Technology Letters 1983

Authors and Affiliations

  • Robert C. Charley
    • 2
  • Jared E. Fein
    • 2
  • Barbara H. Lavers
    • 2
  • Hugh G. Lawford
    • 1
  • G. Ross Lawford
    • 2
  1. 1.Department of BiochemistryUniversity of TorontoTorontoCanada
  2. 2.Weston Research CentreTorontoCanada

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