Biotechnology Letters

, Volume 5, Issue 3, pp 159–164

Ethanol production using Zymomonas mobilis immobilized on an ion exchange resin

  • Thomas A. Krug
  • Andrew J. Daugulis
Article

Summary

Ten ion exchange resins as well as activated carbon and ceramic chips were examined for their ability to adsorb cells of Zymomonas mobilis. A cationic macroreticular resin was shown to be the most efficient adsorbant and was used to immobilize cells of Z. mobilis in a column bioreactor. The bioreactor was operated with a feed glucose concentration of 100 g/L at a dilution rate of 11.2 h-1 and a productivity based on void volume of 377 g ethanol/L-h was obtained with 80% substrate utilization. It was observed that the cell concentration in the bioreactor increased during continuous operation and that the form of Z. mobilis changed from single cells to filamentous forms of the bacterium. Plugging problems occurred after 200 h of operation as a result of excessive filamentous cell growth.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Arcuri, E.J. (1982). Biotech. Bioeng., 24, 595–604.Google Scholar
  2. Bland, R.R., Chen, H.C., Jewell, W.J., Bellamy, W.D., and Zall, R.R. (1982). Biotech. Letters, 4, 5, 323–328.Google Scholar
  3. Daugulis, A.J., Brown, N.M., Cluett, W.R., and Dunlop, B.D. (1981). Biotech. Letters, 3, 11, 651–656.Google Scholar
  4. Margaritis, A., Bajpai, P.K., and Wallace, J.B. (1981). Biotech. Letters.3, 11, 613–618.Google Scholar
  5. Miller, G.L. (1959). Analytical Chemistry. 31, 3, 426–428.Google Scholar
  6. Rogers, P.L., Lee, K.J., Skotnicki, M.L., and Tribe, D.E. (1982). Adv. in Biochem. Eng., 23, 37–84.Google Scholar
  7. Rogers, P.L., Lee, K.J., and Tribe, D.E. (1979). Biotech. Letters, 1, 4, 165–170.Google Scholar
  8. Strandberg, G.W., Donaldson, T.L., and Arcuri, E.J. (1982). Biotech. Letters.4, 6, 347–352.Google Scholar
  9. Swings, J. and De Ley, J. (1977). Bacteriological Reviews, 41, 1, 1–46.Google Scholar

Copyright information

© Science and Technology Letters 1983

Authors and Affiliations

  • Thomas A. Krug
    • 1
  • Andrew J. Daugulis
    • 1
  1. 1.Department of Chemical EngineeringQueen's UniversityKingstonCanada

Personalised recommendations