Biotechnology Letters

, Volume 18, Issue 6, pp 649–654 | Cite as

Studies on the growth, modelling and pigment production by the yeast Phaffia rhodozyma during fed-batch cultivation

  • M. B. Reynders
  • D. E. Rawlings
  • S. T. L. Harrison


As Phaffia rhodozyma is a Crabtree positive yeast, its cell yield and pigment production are reduced at high sugar concentrations. A method for maintaining low growth medium sugar concentrations is fed-batch culture. Using a mass balance approach and Monod growth kinetics a model is presented which describes the fed-batch culture of Phaffia rhodozyma and enables the calculation of a feed regime to obtain the maximum yield of cells and pigment. Although developed on a glucose medium, the model was also applied successfully to a molasses-based medium.


Mass Balance Maximum Yield Growth Kinetic Sugar Concentration Cell Yield 
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Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • M. B. Reynders
    • 1
  • D. E. Rawlings
    • 2
  • S. T. L. Harrison
    • 1
  1. 1.Department of Chemical EngineeringUniversity of Cape TownRondeboschSouth Africa
  2. 2.Department of MicrobiologyUniversity of Cape TownRondeboschSouth Africa

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