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Biotechnology Letters

, Volume 18, Issue 6, pp 649–654 | Cite as

Studies on the growth, modelling and pigment production by the yeast Phaffia rhodozyma during fed-batch cultivation

  • M. B. Reynders
  • D. E. Rawlings
  • S. T. L. Harrison
Article

Summary

As Phaffia rhodozyma is a Crabtree positive yeast, its cell yield and pigment production are reduced at high sugar concentrations. A method for maintaining low growth medium sugar concentrations is fed-batch culture. Using a mass balance approach and Monod growth kinetics a model is presented which describes the fed-batch culture of Phaffia rhodozyma and enables the calculation of a feed regime to obtain the maximum yield of cells and pigment. Although developed on a glucose medium, the model was also applied successfully to a molasses-based medium.

Keywords

Mass Balance Maximum Yield Growth Kinetic Sugar Concentration Cell Yield 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • M. B. Reynders
    • 1
  • D. E. Rawlings
    • 2
  • S. T. L. Harrison
    • 1
  1. 1.Department of Chemical EngineeringUniversity of Cape TownRondeboschSouth Africa
  2. 2.Department of MicrobiologyUniversity of Cape TownRondeboschSouth Africa

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