Biotechnology Letters

, Volume 15, Issue 12, pp 1217–1222 | Cite as

Fermentative production of poly-β-hydroxybutyric acid from xylose by a two-stage culture method employing Lactococcus lactis IO-1 and Alcaligenes eutrophus

  • Kenji Tanaka
  • Kouji Katamune
  • Ayaaki Ishizaki


A two-stage culture method employing Lactococcus.lactis IO-1 and Alcaligenes eutrophus was developed for production of poly-β-hydroxybutyric acid (PHB) from xylose. In this method, xylose was converted to L-lactic acid and acetic acid by L.lactis IO-1, and then the organic acids were converted to PHB by A.eutrophus. When the supernatant of the IO-1 culture broth, containing 10 g·dm-3 L-lactate derived from xylose, was used as medium for A. eutrophus, the concentration of cells increased to 8.5 g·dm-3 in 24 h and 55% of the content in the cells by weight was PHB.


Acetic Acetic Acid Organic Chemistry Xylose Organic Acid 
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Copyright information

© Science and Technology Letters 1993

Authors and Affiliations

  • Kenji Tanaka
    • 1
  • Kouji Katamune
    • 1
  • Ayaaki Ishizaki
    • 1
  1. 1.Department of Food Science and Technology, Faculty of AgricultureKyushu UniversityFukuokaJapan

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