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Biotechnology Letters

, Volume 15, Issue 12, pp 1217–1222 | Cite as

Fermentative production of poly-β-hydroxybutyric acid from xylose by a two-stage culture method employing Lactococcus lactis IO-1 and Alcaligenes eutrophus

  • Kenji Tanaka
  • Kouji Katamune
  • Ayaaki Ishizaki
Article

Summary

A two-stage culture method employing Lactococcus.lactis IO-1 and Alcaligenes eutrophus was developed for production of poly-β-hydroxybutyric acid (PHB) from xylose. In this method, xylose was converted to L-lactic acid and acetic acid by L.lactis IO-1, and then the organic acids were converted to PHB by A.eutrophus. When the supernatant of the IO-1 culture broth, containing 10 g·dm-3 L-lactate derived from xylose, was used as medium for A. eutrophus, the concentration of cells increased to 8.5 g·dm-3 in 24 h and 55% of the content in the cells by weight was PHB.

Keywords

Acetic Acetic Acid Organic Chemistry Xylose Organic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Doi, Y., Tamaki, A., Kunioka, M., & Soga, K. (1987). Makromol. Chem. Rapid Commun., 8, 631Google Scholar
  2. Ishizaki, A. & Tanaka, K. (1991). J. Ferment. Bioeng., 71, 254.Google Scholar
  3. Ishizaki, A., Ohta, T. & Kobayashi, G. (1992a). J. Biotechnology, 24, 85.Google Scholar
  4. Ishizaki, A., Ueda, T., Tanaka, K. & Stanbury, P.F. (1992b). Biotechnology Letters, 14, 599.Google Scholar
  5. Ishizaki,A., Ueda,T., Tanaka,K. & Stanbury, P.F. (1993). Biotechnology Letters, 15, 489.Google Scholar
  6. Linko, S., Vaheri, H., & Sepälä (1993). Appl. Microbiol. Biotechnol., 39, 11.Google Scholar
  7. Mulchandani, A., Luong, H. T. & Groom, C. (1989). Appl. Microbiol. Biotechnol., 30, 11.Google Scholar
  8. Siegel, R.S. & Ollis, D. F. (1984). Biotechnol. Bioeng., 26, 764.Google Scholar
  9. Witholt, B., Smet, M.J., Eggink, G., Kingma, J.& Wynberg, H.(1983). J. Bacteriol., 154, 870.Google Scholar

Copyright information

© Science and Technology Letters 1993

Authors and Affiliations

  • Kenji Tanaka
    • 1
  • Kouji Katamune
    • 1
  • Ayaaki Ishizaki
    • 1
  1. 1.Department of Food Science and Technology, Faculty of AgricultureKyushu UniversityFukuokaJapan

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