Biotechnology Letters

, Volume 17, Issue 6, pp 615–620 | Cite as

Recombinant whole cell penicillin acylase biocatalyst: Production, characterization and use in the synthesis and hydrolysis of antibiotics

  • Sonia Ospina
  • Enrique Merino
  • Octavio T. Ramirez
  • Agustín López-Munguía
Article

The production of a whole cell biocatalyst with high specific penicillin acylase activity using a recombinant strain is described. High activities were obtained using exponentially fed-batch fermentations to overproduce the enzyme. The cells were then entrapped in agar and the activity characterized in terms of particle size and stability. The technical feasibility of the catalyst in synthesis and hydrolysis reactions was demonstrated.

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Copyright information

© Chapman & Hall 1995

Authors and Affiliations

  • Sonia Ospina
    • 1
  • Enrique Merino
    • 2
  • Octavio T. Ramirez
    • 2
  • Agustín López-Munguía
    • 2
  1. 1.Departamento de Farmacia, Instituto de BiotecnologíaUniversidad Nacional de ColombiaSantafé de BogotáColombia
  2. 2.Instituto de Biotecnología, Universidad Nacional Autónoma de MéxicoCuernavaca, Mor.México

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