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Biotechnology Letters

, Volume 15, Issue 5, pp 483–488 | Cite as

Enhanced alpha - amylase production by chromosomal integration of pTVA1 in industrial strain in Bacillus subtilis

  • Slugeňová M. 
  • Bukovská G. 
  • Turňa J. 
  • Timko J. 
Article

Summary

Strain Bacillus subtilis MS was constructed with 12–22 fold increase of α-amylase production, caused by presence of multiple α-amylase gene copies in the chromosome of industrial strain Bacillus subtilis CCM2722, as demonstrated by DNA hybridization. The enhanced production is a result of multiple integration of plasmid pTVA1, carrying a temperature sensitive origin of replication from pE194, and containing the α-amylase gene and a modified transposon Tn917.

Keywords

Organic Chemistry Bacillus Amylase Fold Increase Gene Copy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman & Hall 1993

Authors and Affiliations

  • Slugeňová M. 
    • 1
  • Bukovská G. 
    • 1
  • Turňa J. 
    • 2
    • 1
  • Timko J. 
    • 1
  1. 1.Institute of Molecular Biology, Slovak Academy of ScienceBratislavaSlovak Republic
  2. 2.Department of Molecular BiologyComenius UniversityBratislavaSlovak Republic

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