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Biotechnology Letters

, Volume 16, Issue 6, pp 587–592 | Cite as

Optimization of β-glucosidase catalysed synthesis of trisaccharides from cellobiose and gentiobiose

  • Paul Christakopoulos
  • Dimitris Kekos
  • Basil J. Macris
  • Peter W. Goodenough
  • Mahalingeshwara K. Bhat
Article

Summary

Purified β-Glucosidase from Fusarium oxysporum catalysed the hydrolysis and transglycosylation reactions in the presence of cellobiose and gentiobiose. The product of the latter reaction was mainly a triose. The time of incubation, pH and substrate concentration for transglycosylation reaction were optimised. Under optimal conditions, the concentration of glucose and triose reached approximately 15–20 % of the initial substrate concentration. These results suggested that β-glucosidase from F.oxysporum is an ideal enzyme for the synthesis of triose in reasonable quantities.

Keywords

Glucose Enzyme Hydrolysis Organic Chemistry Optimal Condition 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman & Hall 1994

Authors and Affiliations

  • Paul Christakopoulos
    • 1
  • Dimitris Kekos
    • 2
  • Basil J. Macris
    • 2
  • Peter W. Goodenough
    • 1
  • Mahalingeshwara K. Bhat
    • 1
  1. 1.Department of Protein EngineeringInstitute of Food Research, Reading LaboratoryReadingUK
  2. 2.Department of Chemical EngineeringNational Technical University of AthensAthensGreece

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